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Literature summary extracted from
- Expression of guinea-pig liver transglutaminase cDNA in Escherichia coli. Amino-terminal N alpha-acetyl group is not essential for catalytic function of transglutaminase (1990), Eur. J. Biochem., 187, 705-711.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.3.2.13 | expression in Escherichia coli | Cavia porcellus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.3.2.13 | DTT stabilizes | Cavia porcellus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.3.2.13 | 0.0029 | - |
acetyl-alphaS1-casein | recombinant enzyme | Cavia porcellus | |
| 2.3.2.13 | 0.0032 | - |
acetyl-alphaS1-casein | native enzyme | Cavia porcellus | |
| 2.3.2.13 | 0.04 | 0.05 | putrescine | - |
Cavia porcellus |  |
| 2.3.2.13 | 0.38 | - |
histamine | recombinant enzyme | Cavia porcellus | |
| 2.3.2.13 | 0.52 | - |
histamine | native enzyme | Cavia porcellus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.3.2.13 | Ca2+ | requirement | Cavia porcellus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.3.2.13 | Cavia porcellus | - |
cloned in Escherichia coli | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.3.2.13 | recombinant enzyme, immunoaffinity chromatography, Mono-Q | Cavia porcellus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 2.3.2.13 | liver | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.3.2.13 | 1.2 | - |
recombinant transglutaminase | Cavia porcellus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.3.2.13 | histamine + acetyl-alphaS1-casein | - |
Cavia porcellus | ? | - |
? | |
| 2.3.2.13 | histamine + maleyl-bovine serum albumin | - |
Cavia porcellus | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.3.2.13 | 37 | - |
assay at | Cavia porcellus |
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Release 2023.1 (January 2023)
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