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Literature summary extracted from

  • Martinet, N.; Kim, H.C.; Girard, J.E.; Nigra, D.H.; Strong, D.H.; Chung, S.I.; Folk, J.E.
    Epidermal and hair follicle transglutaminases. Partial characterization of soluble enzymes in newborn mouse skin (1988), J. Biol. Chem., 263, 4236-4241.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.2.13 chromatography on DEAE-cellulose, anionic isozyme, rapid decrease of activity Mus musculus
2.3.2.13 lyophilization, cationic isozyme, stable to Mus musculus
2.3.2.13 repeated freeze-thawing cycles, cationic isozyme, stable to Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ requirement Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 50000
-
 epidermal and hair follicle cationic isozyme, gel filtration Mus musculus
2.3.2.13 90000
-
 epidermis anionic isozyme, gel filtration Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Mus musculus
-
 newborn, CF57 strain
-
2.3.2.13 Mus musculus CF57
-
 newborn, CF57 strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 DEAE-cellulose, CM-cellulose, PO4-cellulose, Sephacryl S-200, Mono-S, cationic dermal isozymes A and B Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.13 epidermis 2 isoforms: anionic and cationic Mus musculus
-
2.3.2.13 hair follicle 2 cationic isoforms Mus musculus
-

Storage Stability

EC Number Storage Stability Organism
2.3.2.13 20°C, pH 6-8, cationic isozyme, long periods Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 putrescine + N,N'-dimethylcasein
-
 Mus musculus ?
-
 ?
2.3.2.13 putrescine + N,N'-dimethylcasein
-
 Mus musculus CF57 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 monomer 1 * 50000, hair follicle transglutaminase, SDS-PAGE Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.13 37
-
 assay at Mus musculus