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Literature summary extracted from

  • Slife, C.W.; Morris, G.S.; Snedeker, S.W.
    Solubilization and properties of the liver plasma membrane transglutaminase (1987), Arch. Biochem. Biophys., 257, 39-47.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.2.13 dithiothreitol
-
 Rattus norvegicus

General Stability

EC Number General Stability Organism
2.3.2.13 Ca2+ stabilizes in combination with dithiothreitol Rattus norvegicus
2.3.2.13 dialysis against Ca2+-free buffers inactivates Rattus norvegicus
2.3.2.13 DTT stabilizes Rattus norvegicus
2.3.2.13 EDTA stabilizes in combination with dithiothreitol Rattus norvegicus
2.3.2.13 glycerol, 50% v/v, solubilizes and stabilizes Rattus norvegicus
2.3.2.13 ion-exchange chromatography on DEAE-cellulose, with 0.4 M NaCl containing buffer with or without Ca2+, stable to Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.13 additional information
-
 additional information kinetic studies of membrane-associated and soluble enzyme Rattus norvegicus
2.3.2.13 additional information
-
 additional information Km of N,N'-dimethylcasein: 0.44-0.52 mg/ml Rattus norvegicus
2.3.2.13 additional information
-
 additional information membrane-associated liver transglutaminase, Km for N,N'-dimethylcasein: 0.44 mg/ml, soluble transglutaminase, 0.52 mg/ml Rattus norvegicus
2.3.2.13 0.04
-
 putrescine soluble liver transglutaminase Rattus norvegicus
2.3.2.13 0.04 0.05 putrescine
-
 Rattus norvegicus
2.3.2.13 0.044
-
 putrescine membrane-associated liver transglutaminase Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.2.13 membrane liver transglutaminase, plasma-membrane associated, lateral domain Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ requirement Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Rattus norvegicus
-
 male Sasco/King (SD)BR strain
-
2.3.2.13 Rattus norvegicus Sasco
-
 male Sasco/King (SD)BR strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 partial Rattus norvegicus
2.3.2.13 selectively solubilized with glycerol Rattus norvegicus
2.3.2.13 Triton X-100, deoxycholate or n-octylglucoside only 20-30% effective Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.13 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.3.2.13 4°C, membrane preparation, rapid inactivation, DTT protects in combination with Ca2+ or EDTA Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 putrescine + N,N'-dimethylcasein
-
 Rattus norvegicus ?
-
 ?
2.3.2.13 putrescine + N,N'-dimethylcasein
-
 Rattus norvegicus Sasco ?
-
 ?