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Literature summary extracted from

  • Wong, W.S.D.; Batt, C.; Kinsella, J.E.
    Purification and characterization of rat liver transglutaminase (1990), Int. J. Biochem., 22, 53-59.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 4-hydroxymercuribenzoate 99% inactivation Rattus norvegicus
2.3.2.13 N-ethylmaleimide 79% inactivation Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.13 0.05
-
 N,N'-dimethylcasein
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ requirement Rattus norvegicus
2.3.2.13 Mg2+ slight activation Rattus norvegicus
2.3.2.13 Mn2+ activation, 3.5% as effective as Ca2+ Rattus norvegicus
2.3.2.13 Sr2+ activation, 27% as effective as Ca2+ Rattus norvegicus
2.3.2.13 Zn2+ activation, 24.6% as effective as Ca2+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 putrescine + N,N'-dimethylcasein
-
 Rattus norvegicus ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.2.13 25
-
 stable at pH 6.0-9.0 Rattus norvegicus
2.3.2.13 44
-
 inactivation within 20 min Rattus norvegicus
2.3.2.13 52
-
 inactivation within 4 min Rattus norvegicus
2.3.2.13 60
-
 inactivation within 1 min Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.2.13 0.532
-
 N,N'-dimethylcasein
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.13 9
-
-
 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.2.13 6 9 stable at 25°C Rattus norvegicus