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Literature summary extracted from

  • Signorini, M.; Bortolotti, F.; Poltronieri, L.; Bergamini, C.M.
    Human erythrocyte transglutaminase: purification and preliminary characterisation (1988), Biol. Chem. Hoppe-Seyler, 369, 275-281.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.2.13 diethyl dicarbonate not without Ca2+ Homo sapiens
2.3.2.13 N-ethylmaleimide strong inactivation in the presence of Ca2+, not inhibited in the absence of Ca2+ Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.2.13 additional information
-
 additional information Km of dimethyl casein: 1.3 mg/ml Homo sapiens
2.3.2.13 0.6
-
 putrescine
-
 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.2.13 Ca2+ requirement Homo sapiens
2.3.2.13 Ga3+ requirement Homo sapiens
2.3.2.13 Mn2+ requirement Homo sapiens
2.3.2.13 Tb3+ requirement Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.2.13 65000
-
 gel filtration and sucrose density gradient centrifugation Homo sapiens
2.3.2.13 92000
-
 1 * 92000, SDS-PAGE with or without 2-mercaptoethanol Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.2.13 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.2.13 erythrocyte transglutaminase, DEAE cellulose, AcA 44, heparin-Sepharose, gel filtration Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.2.13 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.2.13 2.5 3
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.2.13 protein-bound gamma-glutamine + alkylamine substrates are membrane-associated erythrocyte proteins Homo sapiens protein N5-alkylglutamine + NH3
-
 ?
2.3.2.13 putrescine + N,N'-dimethylcasein
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.2.13 monomer 1 * 92000, SDS-PAGE with or without 2-mercaptoethanol Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.2.13 30
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.2.13 additional information
-
 pI: 5.6 Homo sapiens