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Literature summary extracted from

  • Qi, Q.; Rajala, R.V.S.; Anderson, W.; Jiang, C.; Rozwadowski, K.; Selvaraj, G.; Sharma, R.; Datla, R.
    Molecular cloning, genomic organization, and biochemical characterization of myristoyl-CoA:protein N-myristoyltransferase from Arabidopsis thaliana (2000), J. Biol. Chem., 275, 9673-9683.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.97 EDTA 18% activation at 1 mM Arabidopsis thaliana
2.3.1.97 Triton X-100 25% activation at 0.1% Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.97 functional expression in Escherichia coli as His-tagged protein, DNA sequence analysis Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.97 additional information transgenic plants expressing the enzyme under control of the cauliflower mosaic virus 35S promotor, down-regulation by anti-sense expression leads to a growth reduced or lethal phenotype Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.97 factor NIP71 bovine brain inhibitor protein Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.97 additional information
-
 additional information Km for several protein-derived peptides Arabidopsis thaliana
2.3.1.97 0.0205
-
 Arabidopsis thaliana protein CDPK6-derived peptide recombinant enzyme Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.97 cytosol
-
 Arabidopsis thaliana 5829
-
2.3.1.97 mitochondrion
-
 Arabidopsis thaliana 5739
-
2.3.1.97 ribosome major part Arabidopsis thaliana 5840
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.97 additional information
-
 amino acid sequence, alignment Arabidopsis thaliana
2.3.1.97 50000
-
 1 * 50000, recombinant enzyme Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.97 myristoyl-CoA + glycylpeptide Arabidopsis thaliana essential for growth N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + protein Arabidopsis thaliana
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.97 Arabidopsis thaliana Q9LTR9 gene nmt1, 2 gene copies on different chromosomes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.97 recombinant His-tagged enzyme from Escherichia coli Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.97 flower
-
 Arabidopsis thaliana
-
2.3.1.97 leaf
-
 Arabidopsis thaliana
-
2.3.1.97 root
-
 Arabidopsis thaliana
-
2.3.1.97 silique
-
 Arabidopsis thaliana
-
2.3.1.97 stem
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.97 0.00115
-
 purified recombinant enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.97 lauroyl-CoA + glycylpeptide low activity Arabidopsis thaliana N-lauroylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Arabidopsis thaliana protein CDPK6-derived peptide
-
 Arabidopsis thaliana N-myristoylated Arabidopsis thaliana protein CDPK6-derived peptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + Fen kinase-derived peptide plant protein peptide Arabidopsis thaliana N-myristoylated Fen kinase-derived peptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide substrate specificity Arabidopsis thaliana N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide altered fatty acid chain length Arabidopsis thaliana N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide specific for myristoyl-CoA Arabidopsis thaliana N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + glycylpeptide essential for growth Arabidopsis thaliana N-myristoylglycylpeptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + p60src-derived peptide
-
 Arabidopsis thaliana N-myristoylated p60src-derived peptide + CoA
-
 ?
2.3.1.97 myristoyl-CoA + protein
-
 Arabidopsis thaliana ?
-
 ?
2.3.1.97 n-decanoyl-CoA + glycylpeptide low activity Arabidopsis thaliana N-decanoylglycylpeptide + CoA
-
 ?
2.3.1.97 palmitoyl-CoA + glycylpeptide poor substrate Arabidopsis thaliana N-palmitoylglycylpeptide + CoA
-
 ?
2.3.1.97 stearoyl-CoA + glycylpeptide poor substrate Arabidopsis thaliana N-stearoylglycylpeptide + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.97 monomer 1 * 50000, recombinant enzyme Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.3.1.97 NMT
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.97 7.8
-
-
 Arabidopsis thaliana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.97 5.5 8.5
-
 Arabidopsis thaliana