EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.97 | expression in Escherichia coli | Saccharomyces cerevisiae |
2.3.1.97 | expression of mutant nmt1-181 in Escherichia coli | Saccharomyces cerevisiae |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.97 | D451K | site-directed mutagenesis, no functional complementation of enzyme deficient nmt1-181 mutant at 36°C | Saccharomyces cerevisiae |
2.3.1.97 | D451N | site-directed mutagenesis, no functional complementation of enzyme deficient nmt1-181 mutant at 36°C | Saccharomyces cerevisiae |
2.3.1.97 | E167K | site-directed mutagenesis, coexpression of wild-type with Asp451, functional complementation of enzyme deficient nmt1-181 mutant at 36°C | Saccharomyces cerevisiae |
2.3.1.97 | E293K | site-directed mutagenesis, coexpression of wild-type with Asp451, functional complementation of enzyme deficient nmt1-181 mutant at 36°C | Saccharomyces cerevisiae |
2.3.1.97 | additional information | temperature sensitive mutant strain nmt1-181, exchange of Gly to Asp, 10fold increased Km for myristoyl-CoA at 36°C | Saccharomyces cerevisiae |
2.3.1.97 | additional information | disruption or deletion of nmt1-gene causes recessive lethality | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.97 | myristoyl-CoA + glycylpeptide | Saccharomyces cerevisiae | - |
N-myristoylglycylpeptide + CoA | - |
ir | |
2.3.1.97 | myristoyl-CoA + protein | Saccharomyces cerevisiae | natural protein substrates are: catalytic subunit of cAMP-dependent protein kinase, p60src, phosphatases, e.g. calcineurin B, transmembrane signalling proteins, e.g. alpha-subunits of heterotrimeric G proteins, gag polyprotein precursors of several retroviruses, capsid proteins of some parvoviruses and picornaviruses | ? | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.97 | no activity in Escherichia coli | - |
- |
- |
2.3.1.97 | Saccharomyces cerevisiae | - |
gene nmt1 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.97 | myristoyl-CoA + glycylpeptide | - |
Saccharomyces cerevisiae | N-myristoylglycylpeptide + CoA | - |
ir | |
2.3.1.97 | myristoyl-CoA + glycylpeptide | stereochemical requirements | Saccharomyces cerevisiae | N-myristoylglycylpeptide + CoA | - |
ir | |
2.3.1.97 | myristoyl-CoA + glycylpeptide | specific for Gly at N-terminus | Saccharomyces cerevisiae | N-myristoylglycylpeptide + CoA | - |
ir | |
2.3.1.97 | myristoyl-CoA + glycylpeptide | specific for myristoyl-CoA | Saccharomyces cerevisiae | N-myristoylglycylpeptide + CoA | - |
ir | |
2.3.1.97 | myristoyl-CoA + glycylpeptide | p60src-derived peptides | Saccharomyces cerevisiae | N-myristoylglycylpeptide + CoA | - |
ir | |
2.3.1.97 | myristoyl-CoA + protein | natural protein substrates are: catalytic subunit of cAMP-dependent protein kinase, p60src, phosphatases, e.g. calcineurin B, transmembrane signalling proteins, e.g. alpha-subunits of heterotrimeric G proteins, gag polyprotein precursors of several retroviruses, capsid proteins of some parvoviruses and picornaviruses | Saccharomyces cerevisiae | ? | - |
ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.97 | NMT | - |
Saccharomyces cerevisiae |