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Literature summary extracted from

  • Suh, D.Y.; Fukuma, K.; Kagami, J.; Yamazaki, Y.; Shibuya, M.; Ebizuka, Y.; Sankawa, U.
    Identification of amino acid residues important in the cyclization reactions of chalcone and stilbene synthases (2000), Biochem. J., 350, 229-235.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.95 expression of wild-type, P375G and G374L mutant enzyme in Escherichia coli Arachis hypogaea

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.95 G374L no condensing activity Arachis hypogaea
2.3.1.95 P375G product profile of p-coumaroyl condensing reaction changed to: 42% p-coumaroyltriacetic acid, 27% resveratrol, 24% bisnoryangonin and 7.3% naringenin Arachis hypogaea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.95 0.00086
-
malonyl-CoA P375G mutant enzyme Arachis hypogaea
2.3.1.95 0.002
-
malonyl-CoA
-
Arachis hypogaea
2.3.1.95 0.016
-
p-Coumaroyl-CoA P375G mutant enzyme Arachis hypogaea
2.3.1.95 0.022
-
p-Coumaroyl-CoA
-
Arachis hypogaea
2.3.1.95 0.022
-
malonyl-CoA malonyl-CoA decarboxylation, P375G mutant enzyme Arachis hypogaea
2.3.1.95 0.031
-
malonyl-CoA malonyl-CoA decarboxylation Arachis hypogaea

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.95 Arachis hypogaea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.95 recombinant resveratrol synthase, Ni2+-iminodiacetic acid-Sepharose Arachis hypogaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.95 4-coumaroyl-CoA + malonyl-CoA
-
Arachis hypogaea 3,4',5-trihydroxystilbene + CoA + CO2 79% resveratrol, side products: 10% p-coumaroyltriacetic acid lactone, 8.6% bisnoryangonin and 1.7% naringenin ?