EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.95 | expression of wild-type, P375G and G374L mutant enzyme in Escherichia coli | Arachis hypogaea |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.95 | G374L | no condensing activity | Arachis hypogaea |
2.3.1.95 | P375G | product profile of p-coumaroyl condensing reaction changed to: 42% p-coumaroyltriacetic acid, 27% resveratrol, 24% bisnoryangonin and 7.3% naringenin | Arachis hypogaea |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.95 | 0.00086 | - |
malonyl-CoA | P375G mutant enzyme | Arachis hypogaea | |
2.3.1.95 | 0.002 | - |
malonyl-CoA | - |
Arachis hypogaea | |
2.3.1.95 | 0.016 | - |
p-Coumaroyl-CoA | P375G mutant enzyme | Arachis hypogaea | |
2.3.1.95 | 0.022 | - |
p-Coumaroyl-CoA | - |
Arachis hypogaea | |
2.3.1.95 | 0.022 | - |
malonyl-CoA | malonyl-CoA decarboxylation, P375G mutant enzyme | Arachis hypogaea | |
2.3.1.95 | 0.031 | - |
malonyl-CoA | malonyl-CoA decarboxylation | Arachis hypogaea |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.95 | Arachis hypogaea | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.95 | recombinant resveratrol synthase, Ni2+-iminodiacetic acid-Sepharose | Arachis hypogaea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.95 | 4-coumaroyl-CoA + malonyl-CoA | - |
Arachis hypogaea | 3,4',5-trihydroxystilbene + CoA + CO2 | 79% resveratrol, side products: 10% p-coumaroyltriacetic acid lactone, 8.6% bisnoryangonin and 1.7% naringenin | ? |