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Literature summary extracted from

  • Kanayama, N.; Himeda, Y.; Atomi, H.; Ueda, M.; Tanaka, A.
    Expression of acetoacetyl-CoA thiolase isoenzyme genes of n-alkane-assimilating yeast, Candida tropicalis: isoenzymes in two intracellular compartments are derived from the same genes (1997), J. Biochem., 122, 616-621.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.9 expression of cytosolic and peroxisomal thiolase I in Saccharomyces cerevisiae Candida tropicalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.9 0.023
-
 acetoacetyl-CoA recombinant cytosolic thiolase I, thiolysis Candida tropicalis
2.3.1.9 0.025
-
 acetoacetyl-CoA peroxisomal thiolase I, thiolysis Candida tropicalis
2.3.1.9 0.03
-
 CoA peroxisomal thiolase I, thiolysis Candida tropicalis
2.3.1.9 0.032
-
 CoA recombinant peroxisomal thiolase I, thiolysis Candida tropicalis
2.3.1.9 0.037
-
 CoA cytosolic thiolase I, thiolysis Candida tropicalis
2.3.1.9 0.042
-
 acetoacetyl-CoA cytosolic thiolase I, thiolysis Candida tropicalis
2.3.1.9 0.05
-
 CoA recombinant cytosolic thiolase I, thiolysis Candida tropicalis
2.3.1.9 0.057
-
 acetoacetyl-CoA recombinant peroxisomal thiolase I, thiolysis Candida tropicalis
2.3.1.9 0.69
-
 acetyl-CoA recombinant cytosolic thiolase I, thiolysis Candida tropicalis
2.3.1.9 0.74
-
 acetyl-CoA recombinant peroxisomal thiolase I, thiolysis Candida tropicalis
2.3.1.9 0.77
-
 acetyl-CoA cytosolic thiolase I, thiolysis Candida tropicalis
2.3.1.9 1.05
-
 acetyl-CoA peroxisomal thiolase I, thiolysis Candida tropicalis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.9 cytosol cytosolic thiolase I Candida tropicalis 5829
-
2.3.1.9 peroxisome peroxisomal thiolase I Candida tropicalis 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 41000
-
 6 * 41000, cytosolic and peroxisomal thiolase I, SDS-PAGE Candida tropicalis
2.3.1.9 240000
-
 peroxisomal thiolase I, gel filtration Candida tropicalis
2.3.1.9 250000
-
 cytosolic thiolase I, gel filtration Candida tropicalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Candida tropicalis
-
 acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA Rhizobium sp.
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Candida tropicalis
-
 ATCC 20336
-
2.3.1.9 Rhizobium sp.
-
 cicer, strain CC 1192
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 cytosolic thiolase I Candida tropicalis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 59.8
-
-
 Candida tropicalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA
-
 Candida tropicalis acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA
-
 Candida tropicalis acetyl-CoA + acetyl-CoA
-
 r
2.3.1.9 CoA + acetoacetyl-CoA
-
 Rhizobium sp. acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA
-
 Rhizobium sp. acetyl-CoA + acetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.9 hexamer 6 * 41000, cytosolic and peroxisomal thiolase I, SDS-PAGE Candida tropicalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.9 8.1
-
-
 Rhizobium sp.
2.3.1.9 8.1
-
 cytosolic and peroxisomal thiolase I, synthesis Candida tropicalis
2.3.1.9 8.3
-
 cytosolic and peroxisomal thiolase I Candida tropicalis