BRENDA - Enzyme Database

Biosynthetic thiolase from zoogloea ramigera. I. Preliminary characterization and analysis of proton transfer reaction

Davis, J.T.; Moore, R.N.; Imperiali, B.; Pratt, A.J.; Kobayashi, K.; Masamune, S.; Sinskey, A.J.; Walsh, C.T.; Fukui, T.; Tomita, K.; J. Biol. Chem. 262, 82-89 (1987)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.3.1.9
urea: 2.5 M, 17 h, 50% loss of activity, 5 M, 45 min, 50% loss of activity, 7 M, 1 min, 50% loss of activity
Zoogloea ramigera
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.9
iodoacetamide
125fold molar excess, rapid inactivation
Zoogloea ramigera
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.9
0.024
-
acetoacetyl-CoA
-
Zoogloea ramigera
2.3.1.9
0.073
-
acetoacetyl-S-(D-pantetheine) 11-pivalate
-
Zoogloea ramigera
2.3.1.9
0.074
-
acetoacetyl-S-(11-t-butyldimethylsilyl)pantetheine
cosubstrate CoA
Zoogloea ramigera
2.3.1.9
0.12
-
acetoacetyl-S-(11-methoxymethyl)pantetheine
-
Zoogloea ramigera
2.3.1.9
0.21
-
acetoacetyl-10-bis-demethylpantetheine 11-pivalate
-
Zoogloea ramigera
2.3.1.9
0.25
-
acetoacetyl-S-homopantetheine 12-pivalate
-
Zoogloea ramigera
2.3.1.9
0.46
-
acetoacetyl-S-pantetheine
-
Zoogloea ramigera
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.9
162000
-
-
Zoogloea ramigera
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.9
CoA + acetoacetyl-CoA
Zoogloea ramigera
involved in generation of acetoacetyl-CoA for poly-beta-hydroxybutyrate synthesis
acetyl-CoA + acetyl-CoA
-
Zoogloea ramigera
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.9
Zoogloea ramigera
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.9
ammonium sulfate, DEAE-Sepharose, hydroxyapatite, phosphocellulose, Blue Sepharose
Zoogloea ramigera
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.9
0.16
-
acetoacetyl-CoA synthesis
Zoogloea ramigera
2.3.1.9
412
-
thiolysis of acetoacetyl-CoA
Zoogloea ramigera
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.9
CoA + acetoacetyl-10-bis-demethylpantetheine 11-pivaloate
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-10-bis-demethylpantetheine 11-pivaloate
-
487680
Zoogloea ramigera
?
2.3.1.9
CoA + acetoacetyl-CoA
most active in thiolysis
487680
Zoogloea ramigera
acetyl-CoA + acetyl-CoA
-
487680
Zoogloea ramigera
r
2.3.1.9
CoA + acetoacetyl-CoA
involved in generation of acetoacetyl-CoA for poly-beta-hydroxybutyrate synthesis
487680
Zoogloea ramigera
acetyl-CoA + acetyl-CoA
-
487680
Zoogloea ramigera
?
2.3.1.9
CoA + acetoacetyl-S-(11-methoxymethyl)pantetheine
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-S-(11-methoxymethyl)pantetheine
-
-
-
?
2.3.1.9
CoA + acetoacetyl-S-(11-t-butyldimethylsilyl)pantetheine
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-S-(11-t-butyldimethylsilyl)pantetheine
-
487680
Zoogloea ramigera
?
2.3.1.9
CoA + acetoacetyl-S-(D-pantetheine) 11-pivalate
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-S-(D-pantetheine) 11-pivalate
-
487680
Zoogloea ramigera
?
2.3.1.9
CoA + acetoacetyl-S-(L-pantetheine) 11-pivalate
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-S-(L-pantetheine) 11-pivalate
-
487680
Zoogloea ramigera
?
2.3.1.9
CoA + acetoacetyl-S-homopantetheine 12-pivalate
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-S-homopantetheine 12-pivalate
-
487680
Zoogloea ramigera
?
2.3.1.9
CoA + acetoacetyl-S-pantetheine
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-S-pantetheine
-
487680
Zoogloea ramigera
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.9
174
-
acetoacetyl-S-pantetheine
-
Zoogloea ramigera
2.3.1.9
177
-
acetoacetyl-S-homopantetheine 12-pivalate
-
Zoogloea ramigera
2.3.1.9
256
-
acetoacetyl-S-(L-pantetheine) 11-pivalate
-
Zoogloea ramigera
2.3.1.9
266
-
acetoacetyl-10-bis-demethylpantetheine 11-pivaloate
-
Zoogloea ramigera
2.3.1.9
353
-
acetoacetyl-S-(11-methoxymethyl)pantetheine
-
Zoogloea ramigera
2.3.1.9
434
-
acetoacetyl-S-(11-t-butyldimethylsilyl)pantetheine
-
Zoogloea ramigera
2.3.1.9
465
-
acetoacetyl-CoA
-
Zoogloea ramigera
2.3.1.9
469
-
acetoacetyl-S-(D-pantetheine) 11-pivalate
-
Zoogloea ramigera
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.9
urea: 2.5 M, 17 h, 50% loss of activity, 5 M, 45 min, 50% loss of activity, 7 M, 1 min, 50% loss of activity
Zoogloea ramigera
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.9
iodoacetamide
125fold molar excess, rapid inactivation
Zoogloea ramigera
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.9
0.024
-
acetoacetyl-CoA
-
Zoogloea ramigera
2.3.1.9
0.073
-
acetoacetyl-S-(D-pantetheine) 11-pivalate
-
Zoogloea ramigera
2.3.1.9
0.074
-
acetoacetyl-S-(11-t-butyldimethylsilyl)pantetheine
cosubstrate CoA
Zoogloea ramigera
2.3.1.9
0.12
-
acetoacetyl-S-(11-methoxymethyl)pantetheine
-
Zoogloea ramigera
2.3.1.9
0.21
-
acetoacetyl-10-bis-demethylpantetheine 11-pivalate
-
Zoogloea ramigera
2.3.1.9
0.25
-
acetoacetyl-S-homopantetheine 12-pivalate
-
Zoogloea ramigera
2.3.1.9
0.46
-
acetoacetyl-S-pantetheine
-
Zoogloea ramigera
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.9
162000
-
-
Zoogloea ramigera
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.9
CoA + acetoacetyl-CoA
Zoogloea ramigera
involved in generation of acetoacetyl-CoA for poly-beta-hydroxybutyrate synthesis
acetyl-CoA + acetyl-CoA
-
Zoogloea ramigera
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.9
ammonium sulfate, DEAE-Sepharose, hydroxyapatite, phosphocellulose, Blue Sepharose
Zoogloea ramigera
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.9
0.16
-
acetoacetyl-CoA synthesis
Zoogloea ramigera
2.3.1.9
412
-
thiolysis of acetoacetyl-CoA
Zoogloea ramigera
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.9
CoA + acetoacetyl-10-bis-demethylpantetheine 11-pivaloate
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-10-bis-demethylpantetheine 11-pivaloate
-
487680
Zoogloea ramigera
?
2.3.1.9
CoA + acetoacetyl-CoA
most active in thiolysis
487680
Zoogloea ramigera
acetyl-CoA + acetyl-CoA
-
487680
Zoogloea ramigera
r
2.3.1.9
CoA + acetoacetyl-CoA
involved in generation of acetoacetyl-CoA for poly-beta-hydroxybutyrate synthesis
487680
Zoogloea ramigera
acetyl-CoA + acetyl-CoA
-
487680
Zoogloea ramigera
?
2.3.1.9
CoA + acetoacetyl-S-(11-methoxymethyl)pantetheine
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-S-(11-methoxymethyl)pantetheine
-
-
-
?
2.3.1.9
CoA + acetoacetyl-S-(11-t-butyldimethylsilyl)pantetheine
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-S-(11-t-butyldimethylsilyl)pantetheine
-
487680
Zoogloea ramigera
?
2.3.1.9
CoA + acetoacetyl-S-(D-pantetheine) 11-pivalate
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-S-(D-pantetheine) 11-pivalate
-
487680
Zoogloea ramigera
?
2.3.1.9
CoA + acetoacetyl-S-(L-pantetheine) 11-pivalate
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-S-(L-pantetheine) 11-pivalate
-
487680
Zoogloea ramigera
?
2.3.1.9
CoA + acetoacetyl-S-homopantetheine 12-pivalate
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-S-homopantetheine 12-pivalate
-
487680
Zoogloea ramigera
?
2.3.1.9
CoA + acetoacetyl-S-pantetheine
-
487680
Zoogloea ramigera
acetyl-CoA + acetyl-S-pantetheine
-
487680
Zoogloea ramigera
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.3.1.9
174
-
acetoacetyl-S-pantetheine
-
Zoogloea ramigera
2.3.1.9
177
-
acetoacetyl-S-homopantetheine 12-pivalate
-
Zoogloea ramigera
2.3.1.9
256
-
acetoacetyl-S-(L-pantetheine) 11-pivalate
-
Zoogloea ramigera
2.3.1.9
266
-
acetoacetyl-10-bis-demethylpantetheine 11-pivaloate
-
Zoogloea ramigera
2.3.1.9
353
-
acetoacetyl-S-(11-methoxymethyl)pantetheine
-
Zoogloea ramigera
2.3.1.9
434
-
acetoacetyl-S-(11-t-butyldimethylsilyl)pantetheine
-
Zoogloea ramigera
2.3.1.9
465
-
acetoacetyl-CoA
-
Zoogloea ramigera
2.3.1.9
469
-
acetoacetyl-S-(D-pantetheine) 11-pivalate
-
Zoogloea ramigera