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Literature summary extracted from

  • Clinkenbeard, K.D.; Sugiyama, T.; Lane, M.D.
    Cytosolic acetoacetyl-CoA thiolase from chicken liver (1975), Methods Enzymol., 35 B, 167-173.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.9 CoA higher than 0.03 mM, inhibition in cleavage direction Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.9 0.0064
-
 CoA
-
 Gallus gallus
2.3.1.9 0.0385
-
 acetoacetyl-CoA
-
 Gallus gallus
2.3.1.9 0.27
-
 acetyl-CoA
-
 Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.9 cytosol
-
 Gallus gallus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.9 41000
-
 4 * 41000, SDS-PAGE Gallus gallus
2.3.1.9 169000
-
 sedimentation equilibrium Gallus gallus
2.3.1.9 188000
-
 gel filtration Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA Gallus gallus
-
 acetyl-CoA + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.9 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.9 liver enzyme, ammonium sulfate, calcium phosphate gel, phosphocellulose column, DEAE-cellulose, hydroxylapatite, Sephadex G-200 Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.9 liver
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.9 additional information
-
 assay method Gallus gallus
2.3.1.9 203
-
-
 Gallus gallus

Storage Stability

EC Number Storage Stability Organism
2.3.1.9 -90°C, 12 months Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.9 CoA + acetoacetyl-CoA
-
 Gallus gallus acetyl-CoA + acetyl-CoA
-
 ?
2.3.1.9 CoA + acetoacetyl-CoA
-
 Gallus gallus acetyl-CoA + acetyl-CoA
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.9 tetramer 4 * 41000, SDS-PAGE Gallus gallus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.9 30
-
 assay at Gallus gallus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.9 29.5
-
 CoA presence of Mg2+ Gallus gallus
2.3.1.9 900
-
 thiolytic cleavage
-
 Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.9 8.4
-
 cleavage of acetoacetyl-CoA, 85% of maximal activity between pH 8.0 and pH 9.0 Gallus gallus