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Literature summary extracted from

  • Lynen, F.
    Yeast fatty acid synthase (1969), Methods Enzymol., 14, 17-33.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.86 cysteine activation Saccharomyces cerevisiae
2.3.1.86 glutathione activation Saccharomyces cerevisiae

General Stability

EC Number General Stability Organism
2.3.1.86 2-mercaptoethanol, inactivation during storage Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.86 iodoacetamide no inhibition of acetyl transferase activity; pH-independent between 5.0 and 9.0 Saccharomyces cerevisiae
2.3.1.86 N-ethylmaleimide no inhibition of acetyl transferase activity; pH-dependent Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.86 additional information
-
 additional information specific activities for acetyl-CoA derivatives Saccharomyces cerevisiae
2.3.1.86 0.067
-
 NADPH
-
 Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.86 2300000
-
 sedimentation equilibrium Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.86 acetyl-CoA + malonyl-CoA + NADH + NADPH Saccharomyces cerevisiae multifunctional enzyme involved in yeast fat metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.86 Saccharomyces cerevisiae
-
 baker's yeast
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.86
-
 Saccharomyces cerevisiae

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.86 acetyl-CoA + 7 malonyl-CoA + 14 NADPH + 14 H+ = hexadecanoyl-CoA + 7 CoA + 7 CO2 + 14 NADP+ + 7 H2O reaction mechanism Saccharomyces cerevisiae
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.86 1.5 2.5
-
 Saccharomyces cerevisiae

Storage Stability

EC Number Storage Stability Organism
2.3.1.86 -15°C, stable in 90% ammonium sulfate solution containing 0.1 M potassium phosphate, pH 6.5, and 1 mM DTT Saccharomyces cerevisiae
2.3.1.86 4°C, precipitate in 50% ammonium sulfate solution, several days Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.86 acetyl-CoA + 7 malonyl-CoA + 7 NADH + 7 NADPH + 14 H+ S-acetylpantetheine and S-malonylpantetheine and saturated acetyl-CoA derivatives can replace acetyl-CoA and malonyl-CoA Saccharomyces cerevisiae palmitoyl-CoA + 7 CoA + 7 CO2 + 7 NAD+ + 7 NADP+ + 7 H2O palmitoyl-CoA and steraoyl-CoA are main products, myristoyl-CoA is produced in small amounts ?
2.3.1.86 acetyl-CoA + malonyl-CoA + NADH + NADPH multifunctional enzyme involved in yeast fat metabolism Saccharomyces cerevisiae ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.86 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.86 6.5 7
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.86 FMN 4 mol FMN per mol of enzyme complex Saccharomyces cerevisiae
2.3.1.86 NADH requirement, 25% as efficient as NADPH Saccharomyces cerevisiae
2.3.1.86 NADPH requirement Saccharomyces cerevisiae