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Literature summary extracted from

  • Keenan, R.W.; Kruczek, M.E.
    The esterification of dolichol by rat liver microsomes (1976), Biochemistry, 15, 1586-1591.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.83 Triton X-100 1%, several fold activation, inhibition at 3% Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.83 additional information not inhibited by retinol Rattus norvegicus
2.3.1.83 Triton X-100 high concentration, stimulation at low concentration Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.83 0.3
-
 dolichol
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.83 microsome predominant, subcellular distribution Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.83 additional information no Mg2+ required Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.83 Rattus norvegicus
-
 female, Sprague-Dawley strain
-
2.3.1.83 Rattus norvegicus Sprague-Dawley
-
 female, Sprague-Dawley strain
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.83 brain
-
 Rattus norvegicus
-
2.3.1.83 intestinal mucosa
-
 Rattus norvegicus
-
2.3.1.83 kidney
-
 Rattus norvegicus
-
2.3.1.83 liver predominant Rattus norvegicus
-
2.3.1.83 pancreas
-
 Rattus norvegicus
-
2.3.1.83 spleen
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.83 3-sn-phosphatidylcholine + dolichol dolichols with 16-22 isopren units, no substrates are phosphatidylethanolamine, lysophosphatidylcholine, sphingomyelin and palmitoyl-CoA Rattus norvegicus 1-acyl-sn-glycero-3-phosphocholine + acyldolichol
-
 ?
2.3.1.83 3-sn-phosphatidylcholine + dolichol dolichols with 16-22 isopren units, no substrates are phosphatidylethanolamine, lysophosphatidylcholine, sphingomyelin and palmitoyl-CoA Rattus norvegicus Sprague-Dawley 1-acyl-sn-glycero-3-phosphocholine + acyldolichol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.83 38
-
 assay at Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.83 additional information
-
 inactivation at room temperature and above Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.83 6
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.83 4.8 7.5 approx. half-maximal activity at pH 4.8 and 7.5 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.83 6
-
 and below, inactivation Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.83 additional information no requirement for CoA, ATP and Mg2+, separately or together Rattus norvegicus