EC Number | General Stability | Organism |
---|---|---|
2.3.1.8 | 50-60% loss of activity after dialysis for 8 h against 0.05 M Tris buffer, Fe2+ and dithiothreitol stabilize | Gottschalkia acidurici |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.8 | arsenate | 50% inhibition at 10 mM | Gottschalkia acidurici | |
2.3.1.8 | Diethylbarbiturate | - |
Gottschalkia acidurici | |
2.3.1.8 | diphosphate | 50% inhibition at 12 mM | Gottschalkia acidurici | |
2.3.1.8 | additional information | inhibition by various buffer systems | Gottschalkia acidurici | |
2.3.1.8 | phosphate | - |
Gottschalkia acidurici | |
2.3.1.8 | Tris-citrate | - |
Gottschalkia acidurici |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.8 | Fe2+ | required, Fe2+ or Mn2+, Mn2+ is 50-75% as effective as Fe2+ | Gottschalkia acidurici | |
2.3.1.8 | Mn2+ | required, Fe2+ or Mn2+, Mn2+ is 50-75% as effective as Fe2+ | Gottschalkia acidurici | |
2.3.1.8 | additional information | no activity with: Fe3+, Zn2+, Mg2+, Co2+, Ni2+, Sn2+, Cu2+, Mo6+, K+, NH4+ | Gottschalkia acidurici |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.8 | 63000 | 75000 | gel filtration | Gottschalkia acidurici |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.8 | acetyl-CoA + phosphate | Gottschalkia acidurici | - |
CoA + acetyl phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.8 | Gottschalkia acidurici | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.8 | - |
Gottschalkia acidurici |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.8 | 7100 | - |
- |
Gottschalkia acidurici |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.8 | acetyl-CoA + phosphate | - |
Gottschalkia acidurici | CoA + acetyl phosphate | - |
? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.8 | 88 | - |
60 min, 44% loss of activity | Gottschalkia acidurici |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.8 | 8 | 8.1 | - |
Gottschalkia acidurici |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.8 | 7 | 8.7 | pH 7.0: about 10% of activity maximum, pH 8.7: about 20% of activity maximum | Gottschalkia acidurici |