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Literature summary extracted from

  • Lundie, L.L.; Ferry, J.G.
    Activation of acetate by Methanosarcina thermophila. Purification and characterization of phosphotransacetylase (1989), J. Biol. Chem., 264, 18392-18396.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.8 sulfate and phosphate partially protect against heat inactivation Methanosarcina thermophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.8 (NH4)2SO4 above 10 mM Methanosarcina thermophila
2.3.1.8 arsenate
-
 Methanosarcina thermophila
2.3.1.8 Na+
-
 Methanosarcina thermophila
2.3.1.8 phosphate end product inhibitor Methanosarcina thermophila
2.3.1.8 potassium phosphate above 10 mM Methanosarcina thermophila
2.3.1.8 SO42-
-
 Methanosarcina thermophila

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.8 soluble
-
 Methanosarcina thermophila
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.8 K+ K+ or NH4+ at concentration above 10 mM required for maximum activity Methanosarcina thermophila
2.3.1.8 NH4+ above 10 mM Methanosarcina thermophila

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.8 43000
-
 1 * 43000, SDS-PAGE Methanosarcina thermophila
2.3.1.8 52000
-
 gel filtration Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate Methanosarcina thermophila
-
 CoA + acetyl phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.8 Methanosarcina thermophila
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.8 stable to air Methanosarcina thermophila

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.8
-
 Methanosarcina thermophila

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.8 2469
-
-
 Methanosarcina thermophila

Storage Stability

EC Number Storage Stability Organism
2.3.1.8 stable for at least 3 h when stored on ice Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.8 acetyl-CoA + phosphate
-
 Methanosarcina thermophila CoA + acetyl phosphate
-
 r
2.3.1.8 acetyl-CoA + phosphate rate of acetyl-CoA synthesis is 10times greater than rate of acetyl phosphate synthesis Methanosarcina thermophila CoA + acetyl phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.8 monomer 1 * 43000, SDS-PAGE Methanosarcina thermophila

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.8 35 45
-
 Methanosarcina thermophila

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.8 additional information
-
 sulfate and phosphate partially protect against heat inactivation Methanosarcina thermophila
2.3.1.8 70
-
 5 min, stable up to 70°C in absence of additional salts Methanosarcina thermophila
2.3.1.8 80
-
 5 min, complete inactivation in absence of additional salts Methanosarcina thermophila

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.8 7
-
-
 Methanosarcina thermophila