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Literature summary extracted from

  • Bame, K.J.; Rome, L.H.
    Acetyl-CoA: alpha-glucosaminide N-acetyltransferase from rat liver (1987), Methods Enzymol., 138, 607-611.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.78 methyl methanethiolsulfonate activates the membrane-bound enzyme 1.5fold Rattus norvegicus
2.3.1.78 taurodeoxycholate 2 to 3fold increase of activity Rattus norvegicus
2.3.1.78 Triton X-100 2 to 3fold increase of activity Rattus norvegicus

General Stability

EC Number General Stability Organism
2.3.1.78 solubilized enzyme, not stable Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.78 2-mercaptoethanol membrane-bound enzyme is insensitive, solubilized enzyme is inactivated Rattus norvegicus
2.3.1.78 alpha-D-glucosamine 6-phosphate weak Rattus norvegicus
2.3.1.78 alpha-methyl-D-glucose weak Rattus norvegicus
2.3.1.78 cellobiose weak Rattus norvegicus
2.3.1.78 chitin
-
 Rattus norvegicus
2.3.1.78 chitosan
-
 Rattus norvegicus
2.3.1.78 coenzyme A noncompetitive Rattus norvegicus
2.3.1.78 dithiothreitol solubilized protein Rattus norvegicus
2.3.1.78 maltose weak Rattus norvegicus
2.3.1.78 additional information iodoacetamide and N-ethylmaleimide are not inhibitory Rattus norvegicus
2.3.1.78 N-acetyl-alpha-D-glucosamine
-
 Rattus norvegicus
2.3.1.78 N-acetyl-beta-D-glucosamine noncompetitive against acetyl-CoA Rattus norvegicus
2.3.1.78 N-laurylsarcosine 1% Rattus norvegicus
2.3.1.78 p-chloromercuribenzoate
-
 Rattus norvegicus
2.3.1.78 sucrose weak Rattus norvegicus
2.3.1.78 Zwittergent 1% Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.78 0.29 0.3 alpha-D-glucosamine
-
 Rattus norvegicus
2.3.1.78 0.55
-
 acetyl-CoA
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.78 acetyl-CoA + heparan sulfate alpha-D-glucosaminide Rattus norvegicus
-
 CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.78 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.78 acetyl-CoA + heparan sulfate alpha-D-glucosaminide = CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide di-iso ping-pong mechanism Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.78 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.78 -70°C, membrane-bound enzyme, 12 months stable Rattus norvegicus
2.3.1.78 4°C, solubilized enzyme, 25% loss of activity after 7 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.78 acetyl-CoA + alpha-D-glucosamine
-
 Rattus norvegicus CoA + N-acetyl-alpha-D-glucosamine
-
 ?
2.3.1.78 acetyl-CoA + heparan sulfate alpha-D-glucosaminide
-
 Rattus norvegicus CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide
-
 ?
2.3.1.78 acetyl-CoA + heparan sulfate alpha-D-glucosaminide brings about the acetylation of glucosamine groups of heparan sulfate and heparin from which the sulfate has been removed Rattus norvegicus CoA + heparan sulfate N-acetyl-alpha-D-glucosaminide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.78 45
-
 membrane-bound form Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.78 45 55
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.78 37
-
 5 min, solubilized enzyme, 50% loss of activity Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.78 5.5
-
 above, broad maximum Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.78 3 7 coenzyme A
-
 Rattus norvegicus
2.3.1.78 15 17 N-acetyl-alpha-D-glucosamine
-
 Rattus norvegicus