BRENDA - Enzyme Database show

Assay of lecithin-retinol acyltransferase

MacDonald, P.N.; Ong, D.E.; Methods Enzymol. 189, 450-459 (1990)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.3.1.76
medicine
retinyl esterification: storage of vitamin A
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.76
cellular retinol-binding protein
specific inhibition of retinol fatty-acyltransferase by capturing substrate
Rattus norvegicus
2.3.1.76
additional information
not inhibited by PMSF
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.76
microsome
membrane fraction
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.76
acyl-CoA + retinol
Rattus norvegicus
-
CoA + retinyl ester
-
Rattus norvegicus
?
2.3.1.76
acyl-CoA + retinol
Rattus norvegicus Sprague-Dawley
-
CoA + retinyl ester
-
Rattus norvegicus Sprague-Dawley
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.76
Rattus norvegicus
-
Sprague-Dawley
-
2.3.1.76
Rattus norvegicus Sprague-Dawley
-
Sprague-Dawley
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.76
partial purification by differential centrifugation
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.76
small intestine
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.76
additional information
-
activity determined from microsomes
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.76
-70ºC, phosphate buffer, pH 7.2, DTT, 12 months
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.76
acyl-CoA + retinol
-
487493
Rattus norvegicus
CoA + retinyl ester
-
487493
Rattus norvegicus
?
2.3.1.76
acyl-CoA + retinol
-
487493
Rattus norvegicus Sprague-Dawley
CoA + retinyl ester
-
487493
Rattus norvegicus Sprague-Dawley
?
2.3.1.76
palmitoyl-CoA + retinol
-
487493
Rattus norvegicus
CoA + retinylpalmitate
-
487493
Rattus norvegicus
?
2.3.1.76
palmitoyl-CoA + retinol
-
487493
Rattus norvegicus Sprague-Dawley
CoA + retinylpalmitate
-
487493
Rattus norvegicus Sprague-Dawley
?
Application (protein specific)
EC Number
Application
Commentary
Organism
2.3.1.76
medicine
retinyl esterification: storage of vitamin A
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.76
cellular retinol-binding protein
specific inhibition of retinol fatty-acyltransferase by capturing substrate
Rattus norvegicus
2.3.1.76
additional information
not inhibited by PMSF
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.76
microsome
membrane fraction
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.76
acyl-CoA + retinol
Rattus norvegicus
-
CoA + retinyl ester
-
Rattus norvegicus
?
2.3.1.76
acyl-CoA + retinol
Rattus norvegicus Sprague-Dawley
-
CoA + retinyl ester
-
Rattus norvegicus Sprague-Dawley
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.76
partial purification by differential centrifugation
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.76
small intestine
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.76
additional information
-
activity determined from microsomes
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.76
-70ºC, phosphate buffer, pH 7.2, DTT, 12 months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.76
acyl-CoA + retinol
-
487493
Rattus norvegicus
CoA + retinyl ester
-
487493
Rattus norvegicus
?
2.3.1.76
acyl-CoA + retinol
-
487493
Rattus norvegicus Sprague-Dawley
CoA + retinyl ester
-
487493
Rattus norvegicus Sprague-Dawley
?
2.3.1.76
palmitoyl-CoA + retinol
-
487493
Rattus norvegicus
CoA + retinylpalmitate
-
487493
Rattus norvegicus
?
2.3.1.76
palmitoyl-CoA + retinol
-
487493
Rattus norvegicus Sprague-Dawley
CoA + retinylpalmitate
-
487493
Rattus norvegicus Sprague-Dawley
?