BRENDA - Enzyme Database show

Acyl-CoA:retinol acyltransferase and lecithin:retinol acyltransferase activities of bovine retinal pigment epithelial microsomes

Saari, J.C.; Bredberg, D.L.; Methods Enzymol. 190, 156-163 (1990)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
2.3.1.76
medicine
retinyl esterification: storage of vitamin A
Bos taurus
General Stability
EC Number
General Stability
Organism
2.3.1.135
all operation involving retinoids are performed at darkness, or under red light
Rattus norvegicus
2.3.1.135
all operation involving retinoids are performed at darkness, or under red light
Bos taurus
2.3.1.135
buffers are bubbled with argon prior to use
Bos taurus
2.3.1.135
dithiothreitol stabilizes
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.135
0.002
-
all-trans-retinol
-
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.76
microsome
membrane fraction
Bos taurus
-
-
2.3.1.135
microsome
-
Rattus norvegicus
-
-
2.3.1.135
microsome
-
Bos taurus
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.76
acyl-CoA + retinol
Bos taurus
-
CoA + retinyl ester
-
Bos taurus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.76
Bos taurus
-
bovine calf or adult
-
2.3.1.135
Bos taurus
-
-
-
2.3.1.135
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.76
partial purification by differential centrifugation
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.76
neural retina
-
Bos taurus
-
2.3.1.135
intestinal mucosa
-
Rattus norvegicus
-
2.3.1.135
liver
-
Rattus norvegicus
-
2.3.1.135
mammary gland
-
Rattus norvegicus
-
2.3.1.135
retinal pigment epithelium
-
Bos taurus
-
2.3.1.135
testis
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.76
additional information
-
activity determined from microsomes
Bos taurus
2.3.1.135
0.00003
-
intestinal LRAT
Rattus norvegicus
2.3.1.135
0.00006
-
mammary gland LRAT
Rattus norvegicus
2.3.1.135
0.000094
-
testis LRAT
Rattus norvegicus
2.3.1.135
0.000145
-
liver LRAT
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.76
-80ºC, Tris-acetate, pH 7, DTT, 6 months
Bos taurus
2.3.1.135
-80°C, 10 mM Tris acetate, pH 7, 1 mM dithiothreitol, microsomal fraction, stable for at least 6 months
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.76
acyl-CoA + retinol
-
487491
Bos taurus
CoA + retinyl ester
-
487491
Bos taurus
?
2.3.1.76
oleoyl-CoA + retinol
-
487491
Bos taurus
CoA + retinyloleolate
-
487491
Bos taurus
?
2.3.1.76
palmitoyl-CoA + retinol
-
487491
Bos taurus
CoA + retinylpalmitate
-
487491
Bos taurus
?
2.3.1.135
additional information
little or no ability to transfer acyl groups from lysophosphatidylcholine, phosphatidylethanolamine or phosphatidic acid to retinol-(cellular-retinol-binding protein)
487491
Rattus norvegicus
?
-
-
-
-
2.3.1.135
additional information
the fatty acid in the 2-position is important in substrate recognition
487491
Rattus norvegicus
?
-
-
-
-
2.3.1.135
additional information
the fatty acid in the 2-position is important in substrate recognition
487491
Bos taurus
?
-
-
-
-
2.3.1.135
phosphatidylcholine + retinol-(cellular-retinol-binding-protein)
-
487491
Rattus norvegicus
2-acylglycerophosphocholine + retinyl ester-(cellular-retinol-binding-protein)
-
487491
Rattus norvegicus
-
2.3.1.135
phosphatidylcholine + retinol-(cellular-retinol-binding-protein)
-
487491
Bos taurus
2-acylglycerophosphocholine + retinyl ester-(cellular-retinol-binding-protein)
-
487491
Bos taurus
r
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.135
37
-
assay at
Bos taurus
2.3.1.135
37
-
-
Rattus norvegicus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.135
37
-
half-life in absence of substrate: 17 min
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.135
7.5
8
-
Bos taurus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.76
8
-
Tris acetate
Bos taurus
Application (protein specific)
EC Number
Application
Commentary
Organism
2.3.1.76
medicine
retinyl esterification: storage of vitamin A
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.135
all operation involving retinoids are performed at darkness, or under red light
Rattus norvegicus
2.3.1.135
all operation involving retinoids are performed at darkness, or under red light
Bos taurus
2.3.1.135
buffers are bubbled with argon prior to use
Bos taurus
2.3.1.135
dithiothreitol stabilizes
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.135
0.002
-
all-trans-retinol
-
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.76
microsome
membrane fraction
Bos taurus
-
-
2.3.1.135
microsome
-
Rattus norvegicus
-
-
2.3.1.135
microsome
-
Bos taurus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.76
acyl-CoA + retinol
Bos taurus
-
CoA + retinyl ester
-
Bos taurus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.76
partial purification by differential centrifugation
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.76
neural retina
-
Bos taurus
-
2.3.1.135
intestinal mucosa
-
Rattus norvegicus
-
2.3.1.135
liver
-
Rattus norvegicus
-
2.3.1.135
mammary gland
-
Rattus norvegicus
-
2.3.1.135
retinal pigment epithelium
-
Bos taurus
-
2.3.1.135
testis
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.76
additional information
-
activity determined from microsomes
Bos taurus
2.3.1.135
0.00003
-
intestinal LRAT
Rattus norvegicus
2.3.1.135
0.00006
-
mammary gland LRAT
Rattus norvegicus
2.3.1.135
0.000094
-
testis LRAT
Rattus norvegicus
2.3.1.135
0.000145
-
liver LRAT
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.76
-80ºC, Tris-acetate, pH 7, DTT, 6 months
Bos taurus
2.3.1.135
-80°C, 10 mM Tris acetate, pH 7, 1 mM dithiothreitol, microsomal fraction, stable for at least 6 months
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.76
acyl-CoA + retinol
-
487491
Bos taurus
CoA + retinyl ester
-
487491
Bos taurus
?
2.3.1.76
oleoyl-CoA + retinol
-
487491
Bos taurus
CoA + retinyloleolate
-
487491
Bos taurus
?
2.3.1.76
palmitoyl-CoA + retinol
-
487491
Bos taurus
CoA + retinylpalmitate
-
487491
Bos taurus
?
2.3.1.135
additional information
little or no ability to transfer acyl groups from lysophosphatidylcholine, phosphatidylethanolamine or phosphatidic acid to retinol-(cellular-retinol-binding protein)
487491
Rattus norvegicus
?
-
-
-
-
2.3.1.135
additional information
the fatty acid in the 2-position is important in substrate recognition
487491
Rattus norvegicus
?
-
-
-
-
2.3.1.135
additional information
the fatty acid in the 2-position is important in substrate recognition
487491
Bos taurus
?
-
-
-
-
2.3.1.135
phosphatidylcholine + retinol-(cellular-retinol-binding-protein)
-
487491
Rattus norvegicus
2-acylglycerophosphocholine + retinyl ester-(cellular-retinol-binding-protein)
-
487491
Rattus norvegicus
-
2.3.1.135
phosphatidylcholine + retinol-(cellular-retinol-binding-protein)
-
487491
Bos taurus
2-acylglycerophosphocholine + retinyl ester-(cellular-retinol-binding-protein)
-
487491
Bos taurus
r
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.135
37
-
assay at
Bos taurus
2.3.1.135
37
-
-
Rattus norvegicus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
2.3.1.135
37
-
half-life in absence of substrate: 17 min
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.135
7.5
8
-
Bos taurus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.76
8
-
Tris acetate
Bos taurus