BRENDA - Enzyme Database show

Acyl coenzyme A-dependent retinol esterification

Ball, M.D.; Methods Enzymol. 189, 446-449 (1990)

Data extracted from this reference:

Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.76
microsome
membrane fraction
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.76
acyl-CoA + retinol
Rattus norvegicus
-
CoA + retinyl ester
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.76
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.76
partial purification by differential centrifugation
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.76
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.76
additional information
-
activity determined from microsomes; description of assay method
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.76
-70ºC, phosphate buffer, pH 7.4, DTT, 2 months
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.76
acyl-CoA + retinol
-
487489
Rattus norvegicus
CoA + retinyl ester
-
487489
Rattus norvegicus
?
2.3.1.76
palmitoyl-CoA + retinol
polyunsaturated derivatives are poor substrates
487489
Rattus norvegicus
CoA + retinylpalmitate
-
487489
Rattus norvegicus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.76
37
-
assay at
Rattus norvegicus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.76
7.4
-
phosphate buffer
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.76
microsome
membrane fraction
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.76
acyl-CoA + retinol
Rattus norvegicus
-
CoA + retinyl ester
-
Rattus norvegicus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.76
partial purification by differential centrifugation
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.76
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.76
additional information
-
activity determined from microsomes; description of assay method
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.76
-70ºC, phosphate buffer, pH 7.4, DTT, 2 months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.76
acyl-CoA + retinol
-
487489
Rattus norvegicus
CoA + retinyl ester
-
487489
Rattus norvegicus
?
2.3.1.76
palmitoyl-CoA + retinol
polyunsaturated derivatives are poor substrates
487489
Rattus norvegicus
CoA + retinylpalmitate
-
487489
Rattus norvegicus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.76
37
-
assay at
Rattus norvegicus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.76
7.4
-
phosphate buffer
Rattus norvegicus