BRENDA - Enzyme Database show

Retinol esterification by rat liver microsomes. Evidence for a fatty acyl coenzyme A: retinol acyltransferase

Ross, A.C.; J. Biol. Chem. 257, 2453-2459 (1982)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.76
bovine serum albumin
0.02 mM enhances activity
Rattus norvegicus
2.3.1.76
dithiothreitol
5 mM enhances activity
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.76
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
activity inhibited at 0.5%
Rattus norvegicus
2.3.1.76
additional information
not inhibited by cholesterol
Rattus norvegicus
2.3.1.76
p-chloromercuribenzoate
activity abolished at 5 mM
Rattus norvegicus
2.3.1.76
progesterone
activity inhibited above 0.05 mM
Rattus norvegicus
2.3.1.76
sodium deoxycholate
activity inhibited at 0.5%
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.76
microsome
membrane fraction
Felis silvestris
-
-
2.3.1.76
microsome
membrane fraction
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.76
acyl-CoA + retinol
Rattus norvegicus
-
CoA + retinyl ester
-
Rattus norvegicus
?
2.3.1.76
acyl-CoA + retinol
Felis silvestris
-
CoA + retinyl ester
-
Felis silvestris
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.76
Felis silvestris
-
cat
-
2.3.1.76
Rattus norvegicus
-
Sprague-Dawley
-
2.3.1.76
Rattus norvegicus Sprague-Dawley
-
Sprague-Dawley
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.76
partial purification by differential centrifugation
Felis silvestris
2.3.1.76
partial purification by differential centrifugation
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.76
liver
-
Felis silvestris
-
2.3.1.76
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.76
additional information
-
activity determined from microsomes
Felis silvestris
2.3.1.76
additional information
-
activity determined from microsomes
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.76
-70ºC, phosphate buffer, pH 7.4, DTT, 2 months
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.76
acyl-CoA + retinol
-
487488
Rattus norvegicus
CoA + retinyl ester
-
487488
Rattus norvegicus
?
2.3.1.76
acyl-CoA + retinol
-
487488
Felis silvestris
CoA + retinyl ester
-
487488
Felis silvestris
?
2.3.1.76
lauroyl-CoA + retinol
-
487488
Rattus norvegicus
CoA + retinyllaurate
-
487488
Rattus norvegicus
?
2.3.1.76
lauroyl-CoA + retinol
-
487488
Rattus norvegicus Sprague-Dawley
CoA + retinyllaurate
-
487488
Rattus norvegicus Sprague-Dawley
?
2.3.1.76
octanoyl-CoA + retinol
-
487488
Rattus norvegicus
CoA + retinyloctanoate
-
487488
Rattus norvegicus
?
2.3.1.76
octanoyl-CoA + retinol
-
487488
Rattus norvegicus Sprague-Dawley
CoA + retinyloctanoate
-
487488
Rattus norvegicus Sprague-Dawley
?
2.3.1.76
oleoyl-CoA + retinol
-
487488
Rattus norvegicus
CoA + retinyloleolate
-
487488
Rattus norvegicus
?
2.3.1.76
oleoyl-CoA + retinol
-
487488
Rattus norvegicus Sprague-Dawley
CoA + retinyloleolate
-
487488
Rattus norvegicus Sprague-Dawley
?
2.3.1.76
palmitoyl-CoA + retinol
-
487488
Felis silvestris
CoA + retinylpalmitate
-
487488
Felis silvestris
?
2.3.1.76
palmitoyl-CoA + retinol
better effect with long chain fatty acids
487488
Rattus norvegicus
CoA + retinylpalmitate
-
487488
Rattus norvegicus
?
2.3.1.76
palmitoyl-CoA + retinol
better effect with long chain fatty acids
487488
Rattus norvegicus Sprague-Dawley
CoA + retinylpalmitate
-
487488
Rattus norvegicus Sprague-Dawley
?
2.3.1.76
stearoyl-CoA + retinol
-
487488
Rattus norvegicus
CoA + retinylstearate
-
487488
Rattus norvegicus
?
2.3.1.76
stearoyl-CoA + retinol
-
487488
Rattus norvegicus Sprague-Dawley
CoA + retinylstearate
-
487488
Rattus norvegicus Sprague-Dawley
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.76
37
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.76
7
8
maximum esterification
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.76
6
10
maximum esterification between pH 7-8
Rattus norvegicus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.76
7.4
-
phosphate buffer
Felis silvestris
2.3.1.76
7.4
-
phosphate buffer
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.76
bovine serum albumin
0.02 mM enhances activity
Rattus norvegicus
2.3.1.76
dithiothreitol
5 mM enhances activity
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.76
(6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate
activity inhibited at 0.5%
Rattus norvegicus
2.3.1.76
additional information
not inhibited by cholesterol
Rattus norvegicus
2.3.1.76
p-chloromercuribenzoate
activity abolished at 5 mM
Rattus norvegicus
2.3.1.76
progesterone
activity inhibited above 0.05 mM
Rattus norvegicus
2.3.1.76
sodium deoxycholate
activity inhibited at 0.5%
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.76
microsome
membrane fraction
Felis silvestris
-
-
2.3.1.76
microsome
membrane fraction
Rattus norvegicus
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.76
acyl-CoA + retinol
Rattus norvegicus
-
CoA + retinyl ester
-
Rattus norvegicus
?
2.3.1.76
acyl-CoA + retinol
Felis silvestris
-
CoA + retinyl ester
-
Felis silvestris
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.76
partial purification by differential centrifugation
Felis silvestris
2.3.1.76
partial purification by differential centrifugation
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.76
liver
-
Felis silvestris
-
2.3.1.76
liver
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.76
additional information
-
activity determined from microsomes
Felis silvestris
2.3.1.76
additional information
-
activity determined from microsomes
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.76
-70ºC, phosphate buffer, pH 7.4, DTT, 2 months
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.76
acyl-CoA + retinol
-
487488
Rattus norvegicus
CoA + retinyl ester
-
487488
Rattus norvegicus
?
2.3.1.76
acyl-CoA + retinol
-
487488
Felis silvestris
CoA + retinyl ester
-
487488
Felis silvestris
?
2.3.1.76
lauroyl-CoA + retinol
-
487488
Rattus norvegicus
CoA + retinyllaurate
-
487488
Rattus norvegicus
?
2.3.1.76
lauroyl-CoA + retinol
-
487488
Rattus norvegicus Sprague-Dawley
CoA + retinyllaurate
-
487488
Rattus norvegicus Sprague-Dawley
?
2.3.1.76
octanoyl-CoA + retinol
-
487488
Rattus norvegicus
CoA + retinyloctanoate
-
487488
Rattus norvegicus
?
2.3.1.76
octanoyl-CoA + retinol
-
487488
Rattus norvegicus Sprague-Dawley
CoA + retinyloctanoate
-
487488
Rattus norvegicus Sprague-Dawley
?
2.3.1.76
oleoyl-CoA + retinol
-
487488
Rattus norvegicus
CoA + retinyloleolate
-
487488
Rattus norvegicus
?
2.3.1.76
oleoyl-CoA + retinol
-
487488
Rattus norvegicus Sprague-Dawley
CoA + retinyloleolate
-
487488
Rattus norvegicus Sprague-Dawley
?
2.3.1.76
palmitoyl-CoA + retinol
-
487488
Felis silvestris
CoA + retinylpalmitate
-
487488
Felis silvestris
?
2.3.1.76
palmitoyl-CoA + retinol
better effect with long chain fatty acids
487488
Rattus norvegicus
CoA + retinylpalmitate
-
487488
Rattus norvegicus
?
2.3.1.76
palmitoyl-CoA + retinol
better effect with long chain fatty acids
487488
Rattus norvegicus Sprague-Dawley
CoA + retinylpalmitate
-
487488
Rattus norvegicus Sprague-Dawley
?
2.3.1.76
stearoyl-CoA + retinol
-
487488
Rattus norvegicus
CoA + retinylstearate
-
487488
Rattus norvegicus
?
2.3.1.76
stearoyl-CoA + retinol
-
487488
Rattus norvegicus Sprague-Dawley
CoA + retinylstearate
-
487488
Rattus norvegicus Sprague-Dawley
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.3.1.76
37
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.76
7
8
maximum esterification
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.76
6
10
maximum esterification between pH 7-8
Rattus norvegicus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
2.3.1.76
7.4
-
phosphate buffer
Felis silvestris
2.3.1.76
7.4
-
phosphate buffer
Rattus norvegicus