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Literature summary extracted from

  • Ross, A.C.
    Retinol esterification by rat liver microsomes. Evidence for a fatty acyl coenzyme A: retinol acyltransferase (1982), J. Biol. Chem., 257, 2453-2459.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.76 bovine serum albumin 0.02 mM enhances activity Rattus norvegicus
2.3.1.76 dithiothreitol 5 mM enhances activity Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.76 (6R)-6beta-hydroxy-1,4,5,6-tetrahydronicotinamide-adenine dinucleotide phosphate activity inhibited at 0.5% Rattus norvegicus
2.3.1.76 additional information not inhibited by cholesterol Rattus norvegicus
2.3.1.76 p-chloromercuribenzoate activity abolished at 5 mM Rattus norvegicus
2.3.1.76 progesterone activity inhibited above 0.05 mM Rattus norvegicus
2.3.1.76 sodium deoxycholate activity inhibited at 0.5% Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.76 microsome membrane fraction Rattus norvegicus
-
-
2.3.1.76 microsome membrane fraction Felis silvestris
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.76 acyl-CoA + retinol Rattus norvegicus
-
 CoA + retinyl ester
-
 ?
2.3.1.76 acyl-CoA + retinol Felis silvestris
-
 CoA + retinyl ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.76 Felis silvestris
-
 cat
-
2.3.1.76 Rattus norvegicus
-
 Sprague-Dawley
-
2.3.1.76 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.76 partial purification by differential centrifugation Rattus norvegicus
2.3.1.76 partial purification by differential centrifugation Felis silvestris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.76 liver
-
 Rattus norvegicus
-
2.3.1.76 liver
-
 Felis silvestris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.76 additional information
-
 activity determined from microsomes Rattus norvegicus
2.3.1.76 additional information
-
 activity determined from microsomes Felis silvestris

Storage Stability

EC Number Storage Stability Organism
2.3.1.76 -70ºC, phosphate buffer, pH 7.4, DTT, 2 months Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.76 acyl-CoA + retinol
-
 Rattus norvegicus CoA + retinyl ester
-
 ?
2.3.1.76 acyl-CoA + retinol
-
 Felis silvestris CoA + retinyl ester
-
 ?
2.3.1.76 lauroyl-CoA + retinol
-
 Rattus norvegicus CoA + retinyllaurate
-
 ?
2.3.1.76 lauroyl-CoA + retinol
-
 Rattus norvegicus Sprague-Dawley CoA + retinyllaurate
-
 ?
2.3.1.76 octanoyl-CoA + retinol
-
 Rattus norvegicus CoA + retinyloctanoate
-
 ?
2.3.1.76 octanoyl-CoA + retinol
-
 Rattus norvegicus Sprague-Dawley CoA + retinyloctanoate
-
 ?
2.3.1.76 oleoyl-CoA + retinol
-
 Rattus norvegicus CoA + retinyloleolate
-
 ?
2.3.1.76 oleoyl-CoA + retinol
-
 Rattus norvegicus Sprague-Dawley CoA + retinyloleolate
-
 ?
2.3.1.76 palmitoyl-CoA + retinol
-
 Felis silvestris CoA + retinylpalmitate
-
 ?
2.3.1.76 palmitoyl-CoA + retinol better effect with long chain fatty acids Rattus norvegicus CoA + retinylpalmitate
-
 ?
2.3.1.76 palmitoyl-CoA + retinol better effect with long chain fatty acids Rattus norvegicus Sprague-Dawley CoA + retinylpalmitate
-
 ?
2.3.1.76 stearoyl-CoA + retinol
-
 Rattus norvegicus CoA + retinylstearate
-
 ?
2.3.1.76 stearoyl-CoA + retinol
-
 Rattus norvegicus Sprague-Dawley CoA + retinylstearate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.76 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.76 7 8 maximum esterification Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.76 6 10 maximum esterification between pH 7-8 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.76 7.4
-
 phosphate buffer Rattus norvegicus
2.3.1.76 7.4
-
 phosphate buffer Felis silvestris