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Literature summary extracted from

  • Hinderer, W.; Seitz, H.U.
    Chalcone synthase from cell suspension cultures of Daucus carota L (1985), Arch. Biochem. Biophys., 240, 265-272.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.74 2-mercaptoethanol also leads to increased formation of by-products Daucus carota
2.3.1.74 bovine serum albumin
-
 Daucus carota
2.3.1.74 chlorogenic acid increases eriodictyol formation by 20% Daucus carota

General Stability

EC Number General Stability Organism
2.3.1.74 withdrawal of 2-mercaptoethanol after ammonium sulfate precipitation leads to increased stability during further purification Daucus carota

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.74 4-coumaroyl-CoA above 0.01 mM Daucus carota
2.3.1.74 cerulenin
-
 Daucus carota
2.3.1.74 eriodictyol
-
 Daucus carota
2.3.1.74 eriodictyol chalcone
-
 Daucus carota
2.3.1.74 malonyl-CoA substrate inhibition above 0.05 mM Daucus carota
2.3.1.74 naringenin 50% inhibition of naringenin formation and total inhibition of eriodictyol formation Daucus carota
2.3.1.74 naringenin chalcone 50% inhibition of naringenin formation and total inhibition of eriodictyol formation Daucus carota

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.74 0.0006
-
 4-coumaroyl-CoA
-
 Daucus carota
2.3.1.74 0.003
-
 malonyl-CoA
-
 Daucus carota
2.3.1.74 0.0077
-
 caffeoyl-CoA
-
 Daucus carota

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.74 4-coumaroyl-CoA + 3 malonyl-CoA Daucus carota
-
 naringenin chalcone + 4 CoA + 3 CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Daucus carota
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.74 partial Daucus carota

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 cell suspension culture
-
 Daucus carota
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.74 0.162
-
-
 Daucus carota

Storage Stability

EC Number Storage Stability Organism
2.3.1.74 -70°C, decrease of activity in solutions below 1 mg/ml Daucus carota

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 4-coumaroyl-CoA + 3 malonyl-CoA
-
 Daucus carota naringenin chalcone + 4 CoA + 3 CO2
-
 ?
2.3.1.74 caffeoyl-CoA + 3 malonyl-CoA
-
 Daucus carota eriodictyol + 4 CoA + 3 CO2
-
 ?
2.3.1.74 malonyl-CoA + 4-coumaroyl-CoA
-
 Daucus carota naringenin chalcone + CoA + CO2
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.74
-
-
 half-life 3 h in 1.4 mM 2-mercaptoethanol, increase of stability with 14 mM 2-mercaptoethanol Daucus carota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.74 6.8
-
 caffeoyl-CoA Daucus carota
2.3.1.74 7.9
-
 4-coumaroyl-CoA Daucus carota