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Literature summary extracted from

  • Claudot, A.C.; Drouet, A.
    Preparation and assay of chalcone synthase from walnut tree tissue (1992), Phytochemistry, 31, 3377-3380.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.74 2-mercaptoethanol activation Juglans sp.
2.3.1.74 cysteine dependent on Juglans sp.
2.3.1.74 Dowex 77% increase of activity Juglans sp.
2.3.1.74 EDTA 50 mM, 9% increase of activity Juglans sp.
2.3.1.74 N2 79% increase of activity Juglans sp.
2.3.1.74 Polyethyleneglycol activity strongly dependent on 1.5% Juglans sp.
2.3.1.74 Polyvinylpyrrolidone 10% w/v essential Juglans sp.
2.3.1.74 sucrose 25% increase of activity Juglans sp.

General Stability

EC Number General Stability Organism
2.3.1.74 0.1% bovine serum albumin stabilizes Juglans sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.74 4-coumaroyl-CoA substrate inhibition above 0.03 mM Juglans sp.
2.3.1.74 malonyl-CoA
-
 Juglans sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.74 0.0016
-
 4-coumaroyl-CoA
-
 Juglans sp.
2.3.1.74 0.0026
-
 malonyl-CoA
-
 Juglans sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.74 CaCl2 1 mM, 15% increase of activity Juglans sp.

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.74 Juglans sp.
-
 walnut, Juglans nigra * Juglans regia
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.74 sensitive to oxygen Juglans sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.74 bark
-
 Juglans sp.
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.74 -70°C, 0.1% bovine serum albumin, at least 3 weeks Juglans sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.74 malonyl-CoA + 4-coumaroyl-CoA
-
 Juglans sp. naringenin chalcone + CoA + CO2 due to the presence of isomerase the immediate product naringenin chalcone is not detectable, instead 4,2',4',6'-tetrahydroxychalcone is found ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.74 35
-
-
 Juglans sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.74 25 45 less than 40% of maximal activity above and below Juglans sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.74 6.8
-
 assay at Juglans sp.
2.3.1.74 8
-
 4-coumaroyl-CoA Juglans sp.