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Literature summary extracted from

  • Bieber, L.L.; Markwell, M.A.K.
    Peroxisomal and microsomal carnitine acetyltransferases (1981), Methods Enzymol., 71, 351-358.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.7 Clofibrate mitochondrial enzyme increases in specific activity Rattus norvegicus
2.3.1.7 streptozotocin increases enzyme in liver Rattus norvegicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.7
-
 Columba sp.

General Stability

EC Number General Stability Organism
2.3.1.7 enzyme is abolished by detergents, hypotonic media or freeze-thawing Columba sp.
2.3.1.7 very unstable Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.7 0.034
-
 CoASH
-
 Columba sp.
2.3.1.7 0.069
-
 CoASH
-
 Rattus norvegicus
2.3.1.7 0.12
-
 L-(-)-carnitine
-
 Columba sp.
2.3.1.7 0.15
-
 L-(-)-carnitine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.7 endoplasmic reticulum smooth and rough Columba sp. 5783
-
2.3.1.7 microsome
-
 Columba sp.
-
-
2.3.1.7 microsome membrane bound Rattus norvegicus
-
-
2.3.1.7 mitochondrion
-
 Cavia porcellus 5739
-
2.3.1.7 mitochondrion
-
 Rattus norvegicus 5739
-
2.3.1.7 peroxisome
-
 Rattus norvegicus 5777
-
2.3.1.7 soluble
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.7 25000
-
 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus
2.3.1.7 34000
-
 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus
2.3.1.7 51000
-
-
 Columba sp.
2.3.1.7 59000
-
 microsomal and peroxisomal enzyme Rattus norvegicus
2.3.1.7 62000
-
-
 Rattus norvegicus
2.3.1.7 62000
-
-
 Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.7 acetyl-CoA + carnitine Cavia porcellus
-
 O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine Rattus norvegicus
-
 O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine Sus scrofa
-
 O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine Bos taurus
-
 O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine Columba sp.
-
 O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine Cavia porcellus
-
 acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine Rattus norvegicus
-
 acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine Sus scrofa
-
 acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine Bos taurus
-
 acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine Columba sp.
-
 acylcarnitine + CoASH
-
 r
2.3.1.7 acylcarnitine + CoASH Cavia porcellus
-
 acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH Rattus norvegicus
-
 acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH Sus scrofa
-
 acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH Bos taurus
-
 acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH Columba sp.
-
 acyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH Cavia porcellus
-
 acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH Rattus norvegicus
-
 acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH Sus scrofa
-
 acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH Bos taurus
-
 acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH Columba sp.
-
 acetyl-CoA + carnitine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.7 Bos taurus
-
 beef
-
2.3.1.7 Cavia porcellus
-
 guinea pig
-
2.3.1.7 Columba sp.
-
 pigeon
-
2.3.1.7 Rattus norvegicus
-
 rat
-
2.3.1.7 Sus scrofa
-
 pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.7
-
 Bos taurus
2.3.1.7
-
 Columba sp.
2.3.1.7 partial Rattus norvegicus
2.3.1.7 partially Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.7 breast muscle
-
 Columba sp.
-
2.3.1.7 heart
-
 Rattus norvegicus
-
2.3.1.7 heart
-
 Sus scrofa
-
2.3.1.7 heart
-
 Bos taurus
-
2.3.1.7 heart
-
 Columba sp.
-
2.3.1.7 kidney
-
 Rattus norvegicus
-
2.3.1.7 kidney
-
 Sus scrofa
-
2.3.1.7 liver
-
 Cavia porcellus
-
2.3.1.7 liver
-
 Rattus norvegicus
-
2.3.1.7 liver
-
 Columba sp.
-
2.3.1.7 muscle
-
 Rattus norvegicus
-
2.3.1.7 muscle
-
 Columba sp.
-
2.3.1.7 skeletal muscle
-
 Rattus norvegicus
-
2.3.1.7 small intestine
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.7 0.005
-
 liver Rattus norvegicus
2.3.1.7 0.25
-
 skeletal muscle Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.3.1.7 -20°C, peroxisomal and microsomal fractions from isopycnic sucrose density gradients, activity is stable during storage for at least 3 months Rattus norvegicus
2.3.1.7 -20°C, peroxisomes and microsomes stored in 0.4 M KCl, 0.02% sodium azide, 150 mM Tris-HCl, pH, retains at least 95% of their activity when stored for 3 months Rattus norvegicus
2.3.1.7 4°C, enzyme is abolished by prolonged storage Columba sp.
2.3.1.7 4°C, peroxisomal and microsomal fractions from isopycnic sucrose density gradients, activity is stable during storage for 10 days Rattus norvegicus
2.3.1.7 4°C, peroxisomes and microsomes stored in 0.4 M KCl, 0.02% sodium azide, 150 mM Tris-HCl, pH, retains at least 95% of their activity when stored for 18 days Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.7 acetoacetyl-CoA + L-(-)carnitine
-
 Rattus norvegicus O-acetoacetyl(-)carnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine
-
 Cavia porcellus O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine
-
 Rattus norvegicus O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine
-
 Sus scrofa O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine
-
 Bos taurus O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine
-
 Columba sp. O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + L-(-)carnitine
-
 Cavia porcellus O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + L-(-)carnitine
-
 Rattus norvegicus O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + L-(-)carnitine
-
 Sus scrofa O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + L-(-)carnitine
-
 Bos taurus O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + L-(-)carnitine
-
 Columba sp. O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine
-
 Cavia porcellus acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine
-
 Rattus norvegicus acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine
-
 Sus scrofa acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine
-
 Bos taurus acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine
-
 Columba sp. acylcarnitine + CoASH
-
 r
2.3.1.7 acylcarnitine + CoASH
-
 Cavia porcellus acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH
-
 Rattus norvegicus acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH
-
 Sus scrofa acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH
-
 Bos taurus acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH
-
 Columba sp. acyl-CoA + carnitine
-
 r
2.3.1.7 additional information
-
 Sus scrofa ?
-
 ?
2.3.1.7 additional information
-
 Columba sp. ?
-
 ?
2.3.1.7 additional information O-acetyl-(+)-carnitine and reduced glutathione will not serve as substrates, succinyl-CoA or beta-hydroxy-betamethylglutaryl-CoA are no substrates Rattus norvegicus ?
-
 ?
2.3.1.7 O-acetoacetyl(-)carnitine + CoASH
-
 Rattus norvegicus acetoacetyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Cavia porcellus acetyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Rattus norvegicus acetyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Sus scrofa acetyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Bos taurus acetyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Columba sp. acetyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Cavia porcellus acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Rattus norvegicus acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Sus scrofa acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Bos taurus acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Columba sp. acetyl-CoA + carnitine
-
 r
2.3.1.7 propionyl-CoA + L-(-)carnitine
-
 Rattus norvegicus propionylcarnitine + CoASH
-
 r
2.3.1.7 propionyl-CoA + L-(-)carnitine
-
 Bos taurus propionylcarnitine + CoASH
-
 r
2.3.1.7 propionylcarnitine + CoASH
-
 Rattus norvegicus propionyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 propionylcarnitine + CoASH
-
 Bos taurus propionyl-CoA + L-(-)carnitine
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.7 dimer 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.7 6 7.5 microsomal transferase is more stable at pH 7.5 than at pH 6.0 Rattus norvegicus