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Literature summary extracted from

  • Mittal, B.; Kurup, C.K.R.
    Purification of clofibrate-induced carnitine acetyltransferase from rat liver mitochondria (1980), Biochim. Biophys. Acta, 619, 90-97.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.7 Clofibrate
-
 Rattus norvegicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.7
-
 Columba sp.

General Stability

EC Number General Stability Organism
2.3.1.7 retains activity indefinitely when kept frozen, pure enzyme retains activity when kept frozen for long periods of time, inactivation of pure preparations occurs during dialysis Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.7 acetylcarnitine competitive inhibition, inhibitor for the forward reaction Rattus norvegicus
2.3.1.7 acetylcarnitine
-
 Sus scrofa
2.3.1.7 DL-carnitine inhibitor for the reverse reaction Rattus norvegicus
2.3.1.7 iodoacetamide
-
 Rattus norvegicus
2.3.1.7 iodoacetamide
-
 Sus scrofa
2.3.1.7 N-ethylmaleimide
-
 Rattus norvegicus
2.3.1.7 N-ethylmaleimide
-
 Sus scrofa
2.3.1.7 p-hydroxymercuribenzoate
-
 Rattus norvegicus
2.3.1.7 p-hydroxymercuribenzoate
-
 Sus scrofa
2.3.1.7 palmitoyl-CoA
-
 Columba sp.
2.3.1.7 palmitoyl-CoA
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.7 0.03
-
 CoASH
-
 Rattus norvegicus
2.3.1.7 0.037
-
 CoASH
-
 Columba sp.
2.3.1.7 0.038
-
 butyryl-CoA
-
 Rattus norvegicus
2.3.1.7 0.12
-
 L-(-)-carnitine
-
 Columba sp.
2.3.1.7 0.28
-
 acetylcarnitine
-
 Rattus norvegicus
2.3.1.7 0.72
-
 DL-carnitine
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.7 microsome
-
 Columba sp.
-
-
2.3.1.7 microsome membrane bound Rattus norvegicus
-
-
2.3.1.7 mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.7 25000
-
 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus
2.3.1.7 34000
-
 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus
2.3.1.7 55000
-
 gel filtration Columba sp.
2.3.1.7 56000
-
 gel filtration Rattus norvegicus
2.3.1.7 75000
-
 SDS-PAGE Columba sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.7 acetyl-CoA + carnitine Rattus norvegicus
-
 O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine Sus scrofa
-
 O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine Columba sp.
-
 O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine Rattus norvegicus
-
 acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine Sus scrofa
-
 acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine Columba sp.
-
 acylcarnitine + CoASH
-
 r
2.3.1.7 acylcarnitine + CoASH Rattus norvegicus
-
 acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH Sus scrofa
-
 acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH Columba sp.
-
 acyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH Rattus norvegicus
-
 acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH Sus scrofa
-
 acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH Columba sp.
-
 acetyl-CoA + carnitine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.7 Columba sp.
-
-
-
2.3.1.7 Rattus norvegicus
-
 rat
-
2.3.1.7 Rattus norvegicus
-
 albino
-
2.3.1.7 Sus scrofa
-
 swine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.7
-
 Rattus norvegicus
2.3.1.7
-
 Columba sp.
2.3.1.7 partially Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.7 breast muscle
-
 Columba sp.
-
2.3.1.7 heart
-
 Rattus norvegicus
-
2.3.1.7 heart
-
 Sus scrofa
-
2.3.1.7 heart
-
 Columba sp.
-
2.3.1.7 liver
-
 Rattus norvegicus
-
2.3.1.7 liver
-
 Columba sp.
-
2.3.1.7 muscle
-
 Rattus norvegicus
-
2.3.1.7 muscle
-
 Columba sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.7 82.83
-
-
 Rattus norvegicus
2.3.1.7 85
-
-
 Rattus norvegicus
2.3.1.7 85
-
-
 Columba sp.

Storage Stability

EC Number Storage Stability Organism
2.3.1.7 0-4°C, loses little activity when stored for months Rattus norvegicus
2.3.1.7 0-4°C, loses little activity when stored for months Columba sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.7 acetyl-CoA + carnitine
-
 Rattus norvegicus O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine
-
 Sus scrofa O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + carnitine
-
 Columba sp. O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + L-(-)carnitine
-
 Rattus norvegicus O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + L-(-)carnitine
-
 Sus scrofa O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acetyl-CoA + L-(-)carnitine
-
 Columba sp. O-acetylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine
-
 Rattus norvegicus acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine
-
 Sus scrofa acylcarnitine + CoASH
-
 r
2.3.1.7 acyl-CoA + carnitine
-
 Columba sp. acylcarnitine + CoASH
-
 r
2.3.1.7 acylcarnitine + CoASH
-
 Rattus norvegicus acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH
-
 Sus scrofa acyl-CoA + carnitine
-
 r
2.3.1.7 acylcarnitine + CoASH
-
 Columba sp. acyl-CoA + carnitine
-
 r
2.3.1.7 additional information
-
 Sus scrofa ?
-
 ?
2.3.1.7 additional information
-
 Columba sp. ?
-
 ?
2.3.1.7 additional information palmityl-CoA does not act as a substrate for the liver enzyme Rattus norvegicus ?
-
 ?
2.3.1.7 O-acetylcarnitine + CoASH
-
 Rattus norvegicus acetyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Sus scrofa acetyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Columba sp. acetyl-CoA + L-(-)carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Rattus norvegicus acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Sus scrofa acetyl-CoA + carnitine
-
 r
2.3.1.7 O-acetylcarnitine + CoASH
-
 Columba sp. acetyl-CoA + carnitine
-
 r

Subunits

EC Number Subunits Comment Organism
2.3.1.7 dimer 1 * 34000 + 1 * 25000, purified from mitochondrial fraction of liver, rats fed on a diet containing ethyl p-chlorophenoxyisobutyrate, SDS-PAGE Rattus norvegicus
2.3.1.7 monomer
-
 Rattus norvegicus
2.3.1.7 monomer 1 * 75000, SDS-PAGE Columba sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.7 37
-
-
 Rattus norvegicus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.7 20 40
-
 Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.7 37 50 enzyme retains total activity when kept at 37°C for 30 min, rapid loss of activity above 37°C, loses half of the activity at 40°C for 30 min, but is completely inactivated when kept at 44°C for 30 min or 50°C for 5 min, 75% loss of activity at 37°C for 8 h, only 15% loss when kept at 25°C for 8 h Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.7 0.2
-
 DL-carnitine
-
 Rattus norvegicus
2.3.1.7 8
-
 palmityl-CoA substrate carnitine Rattus norvegicus
2.3.1.7 18.6
-
 palmityl-CoA substrate acetyl-CoA Rattus norvegicus
2.3.1.7 920
-
 acetylcarnitine substrate DL-carnitine Rattus norvegicus