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Literature summary extracted from

  • Wykle, R.L.; Malone, B.; Snyder, F.
    Enzymatic synthesis of 1-alkyl-2-acetyl-sn-glycero-3-phosphocholine, a hypotensive and platelet-aggregating lipid (1980), J. Biol. Chem., 255, 10256-10260.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.67 CaCl2 15% inhibition Rattus norvegicus
2.3.1.67 diisopropylfluorophosphate 98% inhibition at 10 mM Rattus norvegicus
2.3.1.67 EGTA
-
 Rattus norvegicus
2.3.1.67 MgCl2 98% inhibition Rattus norvegicus
2.3.1.67 MnCl2 50% inhibition Rattus norvegicus
2.3.1.67 p-bromophenacyl bromide 90% inhibition at 0.1 mM Rattus norvegicus
2.3.1.67 palmitoyl-CoA 85% inhibition at 10 uM due to detergent effect Rattus norvegicus
2.3.1.67 Triton X-100 75 and 95% inhibition at 0.2 and 0.6 mM respectively due to detergent Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.67 0.067
-
 acetyl-CoA
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.67 microsome
-
 Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.67 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.67 bone marrow
-
 Rattus norvegicus
-
2.3.1.67 liver
-
 Rattus norvegicus
-
2.3.1.67 lung
-
 Rattus norvegicus
-
2.3.1.67 lymph node
-
 Rattus norvegicus
-
2.3.1.67 renal cortex
-
 Rattus norvegicus
-
2.3.1.67 renal medulla
-
 Rattus norvegicus
-
2.3.1.67 spleen
-
 Rattus norvegicus
-
2.3.1.67 thymus
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.67 0.0002
-
 liver Rattus norvegicus
2.3.1.67 0.0002
-
 kidney cortex Rattus norvegicus
2.3.1.67 0.002
-
 kidney medulla Rattus norvegicus
2.3.1.67 0.0026
-
 bone marrow Rattus norvegicus
2.3.1.67 0.0039
-
 lymph nodes Rattus norvegicus
2.3.1.67 0.004
-
 lung Rattus norvegicus
2.3.1.67 0.004
-
 thymus Rattus norvegicus
2.3.1.67 0.01
-
 spleen microsome Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.67 acetyl-CoA + 1-palmitoyl-2-lyso-sn-glycero-3-phosphocholine rate of synthesis is 55% of the rate observed with the ether linked substrate Rattus norvegicus CoA + 2-acetyl-1-palmitoyl-sn-glycero-3-phosphocholine
-
 ?
2.3.1.67 acetyl-CoA + acyl-lyso-glycero-3-phosphocholine worse substrate than alkyl-lyso-glycero-3-phosphocholine Rattus norvegicus CoA + acetyl-acyl-glycero-3-phosphocholine
-
 ?
2.3.1.67 acetyl-CoA + alkyl-sn-glycero-3-phosphocholine
-
 Rattus norvegicus CoA + 2-acetyl-1-alkyl-sn-glycero-3-phosphocholine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.67 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.67 6.9
-
 assay at Rattus norvegicus