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Literature summary extracted from

  • Bird, C.R.; Smith, T.A.
    Agmatine coumaroyltransferase (barley seedlings) (1983), Methods Enzymol., 94, 344-347.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.64 0.00074
-
4-coumaroyl-CoA
-
Hordeum vulgare
2.3.1.64 0.012
-
agmatine
-
Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.64 40000
-
gel filtration Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.64 Hordeum vulgare
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.64 ammonium sulfate, Biogel A, Sepharose 4B-agmatine Hordeum vulgare

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.64 seedling shoots, not seeds Hordeum vulgare
-
2.3.1.64 seedling maximal activity 3-4 days after germination at 22°C in the dark Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.64 0.393
-
-
Hordeum vulgare

Storage Stability

EC Number Storage Stability Organism
2.3.1.64 -40°C, concentrated preparation at least 1 month, negligible loss of activity Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.64 4-coumaroyl-CoA + agmatine no activity with putrescine, spermidine, spermine, cadaverine, arginine, homoarginine Hordeum vulgare CoA + 4-coumaroylagmatine
-
?
2.3.1.64 caffeoyl-CoA + agmatine
-
Hordeum vulgare CoA + caffeoylagmatine
-
?
2.3.1.64 feruloyl-CoA + agmatine
-
Hordeum vulgare CoA + feruloyl agmatine
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.64 7.5
-
-
Hordeum vulgare

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.64 7 8 approx. half-maximal activity at pH 7.0 and 8.0 Hordeum vulgare