BRENDA - Enzyme Database

Aminoglycoside resistance in bacteria medicated by gentamicin acetyltransferase II, an enzyme modifying the 2-amino group of aminoglycoside antibiotics

Chevereau, M.; Daniels, P.J.L.; Davies, J.; LeGoffic, F.; Biochemistry 13, 598-603 (1974)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.3.1.59
activity in crude enzyme preparation is stable to repeated freezing and thawing
Providencia sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.59
Providencia sp.
-
clinical isolate
-
2.3.1.59
Providencia sp. 164
-
clinical isolate
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.59
acetyl-CoA + butirosin
acetylated at 30% the rate of gentamicin C1
487273
Providencia sp.
CoA + ?
-
-
-
?
2.3.1.59
acetyl-CoA + dideoxykanamycin
acetylated at 92% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-diacetyldideoxykanamycin
-
-
-
?
2.3.1.59
acetyl-CoA + gentamicin B
7% of the activity with gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylgentamycin
-
-
-
?
2.3.1.59
acetyl-CoA + gentamicin C1
best substrate
487273
Providencia sp.
CoA + N2'-acetylgentamicin C1
-
-
-
?
2.3.1.59
acetyl-CoA + gentamicin C1
best substrate
487273
Providencia sp. 164
CoA + N2'-acetylgentamicin C1
-
-
-
?
2.3.1.59
acetyl-CoA + gentamicin C1a
acetylated at 86% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylgentamicin C1a
-
-
-
?
2.3.1.59
acetyl-CoA + gentamicin C1a
acetylated at 86% the rate of gentamicin C1
487273
Providencia sp. 164
CoA + N2'-acetylgentamicin C1a
-
-
-
?
2.3.1.59
acetyl-CoA + kanamycin B
acetylated at 14% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2-acetylkanamycin B
-
-
-
?
2.3.1.59
acetyl-CoA + kanamycin C
acetylated at 40% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylkanamycin C
-
-
-
?
2.3.1.59
acetyl-CoA + neomycin B
acetylated at 10% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylneomycin
-
-
-
?
2.3.1.59
acetyl-CoA + ribostamycin
acetylated at 15% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylribostamycin
-
-
-
?
2.3.1.59
acetyl-CoA + sisomicin
acetylated at 71% the rate of gentamicin C1
487273
Providencia sp.
CoA + N3'-acetylsisomicin
-
-
-
-
2.3.1.59
acetyl-CoA + sisomicin
acetylated at 71% the rate of gentamicin C1
487273
Providencia sp. 164
CoA + N3'-acetylsisomicin
-
-
-
-
2.3.1.59
acetyl-CoA + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetyltobramycin
-
-
-
?
2.3.1.59
acetyl-CoA + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp. 164
CoA + N2'-acetyltobramycin
-
-
-
?
2.3.1.59
acetyl-CoA of soluble fragments of peptidoglycan + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp.
?
-
-
-
?
2.3.1.59
additional information
no substrates are kanamycin A and 1-hydroxyaminobutyric acid kanamycin A-derivative BB-K 8
487273
Providencia sp.
?
-
-
-
-
2.3.1.59
additional information
no substrates are kanamycin A and 1-hydroxyaminobutyric acid kanamycin A-derivative BB-K 8
487273
Providencia sp. 164
?
-
-
-
-
2.3.1.59
N-acetyl-D-glucosamine + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp.
D-glucosamine + N2'-acetyltobramycin
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.59
6
-
kanamycin C
Providencia sp.
2.3.1.59
6.6
-
gentamicin C
Providencia sp.
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.59
5.7
6.5
about half-maximal activity at pH 5.7 and 6.5, kanamycin C
Providencia sp.
2.3.1.59
6.4
7.6
about half-maximal activity at pH 6.4 and 7.6, gentamicin C
Providencia sp.
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.59
activity in crude enzyme preparation is stable to repeated freezing and thawing
Providencia sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.59
acetyl-CoA + butirosin
acetylated at 30% the rate of gentamicin C1
487273
Providencia sp.
CoA + ?
-
-
-
?
2.3.1.59
acetyl-CoA + dideoxykanamycin
acetylated at 92% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-diacetyldideoxykanamycin
-
-
-
?
2.3.1.59
acetyl-CoA + gentamicin B
7% of the activity with gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylgentamycin
-
-
-
?
2.3.1.59
acetyl-CoA + gentamicin C1
best substrate
487273
Providencia sp.
CoA + N2'-acetylgentamicin C1
-
-
-
?
2.3.1.59
acetyl-CoA + gentamicin C1
best substrate
487273
Providencia sp. 164
CoA + N2'-acetylgentamicin C1
-
-
-
?
2.3.1.59
acetyl-CoA + gentamicin C1a
acetylated at 86% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylgentamicin C1a
-
-
-
?
2.3.1.59
acetyl-CoA + gentamicin C1a
acetylated at 86% the rate of gentamicin C1
487273
Providencia sp. 164
CoA + N2'-acetylgentamicin C1a
-
-
-
?
2.3.1.59
acetyl-CoA + kanamycin B
acetylated at 14% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2-acetylkanamycin B
-
-
-
?
2.3.1.59
acetyl-CoA + kanamycin C
acetylated at 40% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylkanamycin C
-
-
-
?
2.3.1.59
acetyl-CoA + neomycin B
acetylated at 10% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylneomycin
-
-
-
?
2.3.1.59
acetyl-CoA + ribostamycin
acetylated at 15% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetylribostamycin
-
-
-
?
2.3.1.59
acetyl-CoA + sisomicin
acetylated at 71% the rate of gentamicin C1
487273
Providencia sp.
CoA + N3'-acetylsisomicin
-
-
-
-
2.3.1.59
acetyl-CoA + sisomicin
acetylated at 71% the rate of gentamicin C1
487273
Providencia sp. 164
CoA + N3'-acetylsisomicin
-
-
-
-
2.3.1.59
acetyl-CoA + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp.
CoA + N2'-acetyltobramycin
-
-
-
?
2.3.1.59
acetyl-CoA + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp. 164
CoA + N2'-acetyltobramycin
-
-
-
?
2.3.1.59
acetyl-CoA of soluble fragments of peptidoglycan + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp.
?
-
-
-
?
2.3.1.59
additional information
no substrates are kanamycin A and 1-hydroxyaminobutyric acid kanamycin A-derivative BB-K 8
487273
Providencia sp.
?
-
-
-
-
2.3.1.59
additional information
no substrates are kanamycin A and 1-hydroxyaminobutyric acid kanamycin A-derivative BB-K 8
487273
Providencia sp. 164
?
-
-
-
-
2.3.1.59
N-acetyl-D-glucosamine + tobramycin
acetylated at 58% the rate of gentamicin C1
487273
Providencia sp.
D-glucosamine + N2'-acetyltobramycin
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.59
6
-
kanamycin C
Providencia sp.
2.3.1.59
6.6
-
gentamicin C
Providencia sp.
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.59
5.7
6.5
about half-maximal activity at pH 5.7 and 6.5, kanamycin C
Providencia sp.
2.3.1.59
6.4
7.6
about half-maximal activity at pH 6.4 and 7.6, gentamicin C
Providencia sp.