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Literature summary extracted from

  • Wittich, R.M.; Walter, R.D.
    A novel type of putrescine (diamine)-acetylating enzyme from the nematode Ascaris suum (1989), Biochem. J., 260, 265-269.
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
2.3.1.57 PMSF and Brij 35 are essential to prevent rapid loss of activity of the enzyme preparation Ascaris suum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.57 additional information not inhibited by CoA Ascaris suum
2.3.1.57 N-acetylputrescine slight, competitive inhibition Ascaris suum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.57 0.0077
-
 acetyl-CoA
-
 Ascaris suum
2.3.1.57 0.25
-
 putrescine
-
 Ascaris suum
2.3.1.57 0.4
-
 1,6-diaminohexane
-
 Ascaris suum
2.3.1.57 1.25
-
 1,3-diaminopropane
-
 Ascaris suum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.57 cytosol
-
 Ascaris suum 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.57 13000
-
 x * ? + x * 13000, catalytic subunit, at least 2 subunits, gel filtration of enzyme precipitated by (NH4)2SO4 Ascaris suum
2.3.1.57 38000 40000 gel filtration Ascaris suum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.57 additional information Ascaris suum important function in the degradation of diamines of lower eukaryotes ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.57 Ascaris suum
-
 female adults
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.57 1027fold Ascaris suum
2.3.1.57 partial Ascaris suum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.57 ovary
-
 Ascaris suum
-
2.3.1.57 uterus
-
 Ascaris suum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.57 0.4314
-
-
 Ascaris suum

Storage Stability

EC Number Storage Stability Organism
2.3.1.57 -22°C, per month, 10-16% loss of activity Ascaris suum
2.3.1.57 4°C, per day, 5-9% loss of activity Ascaris suum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.57 acetyl-CoA + 1,3-diaminopropane
-
 Ascaris suum CoA + N1-acetyl-1,3-diaminopropane
-
 ir
2.3.1.57 acetyl-CoA + 1,5-diaminopentane cadaverine Ascaris suum CoA + N1-acetyl-1,5-diaminopentane
-
 ir
2.3.1.57 acetyl-CoA + 1,6-diaminohexane
-
 Ascaris suum CoA + N1-acetyl-1,6-diaminohexane
-
 ir
2.3.1.57 acetyl-CoA + putrescine
-
 Ascaris suum CoA + N-acetylputrescine
-
 ?
2.3.1.57 acetyl-CoA + putrescine
-
 Ascaris suum CoA + N-acetylputrescine
-
 ir
2.3.1.57 acetyl-CoA + spermidine very poor substrate, at 2-4% of the rate with putrescine Ascaris suum CoA + N1-acetylspermidine
-
 ?
2.3.1.57 acetyl-CoA + spermine very poor substrate, at 2-4% of the rate with putrescine Ascaris suum CoA + N1-acetylspermine
-
 ?
2.3.1.57 acetyl-CoA + sym-norspermidine
-
 Ascaris suum CoA + N1-acetyl-sym-norspermidine
-
 ir
2.3.1.57 acetyl-CoA + sym-norspermine at a much lower rate than with sym-norspermidine Ascaris suum CoA + N1-acetyl-sym-norspermine
-
 ir
2.3.1.57 additional information not: histone Ascaris suum ?
-
 ?
2.3.1.57 additional information important function in the degradation of diamines of lower eukaryotes Ascaris suum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.57 ? x * ? + x * 13000, catalytic subunit, at least 2 subunits, gel filtration of enzyme precipitated by (NH4)2SO4 Ascaris suum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.57 37
-
 assay at Ascaris suum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.57 37
-
 half-life: above 1 h Ascaris suum
2.3.1.57 56
-
 half-life: less than 0.5 min Ascaris suum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.57 7.7 7.9
-
 Ascaris suum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.57 7.6 8 high conversion rates in the range of Ascaris suum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.57 1.7
-
 N-acetylputrescine
-
 Ascaris suum