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Literature summary extracted from

  • Asanuma, N.; Hino, T.
    Molecular characterization and expression of pyruvate formate-lyase-activating enzyme in a ruminal bacterium, Streptococcus bovis (2002), Appl. Environ. Microbiol., 68, 3352-3357.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.97.1.4
-
 Streptococcus equinus
2.3.1.54
-
 Lactococcus lactis
2.3.1.54
-
 Streptococcus equinus
2.3.1.54
-
 Clostridium pasteurianum
2.3.1.54 recombinant pfl in Escherichia coli RM220 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.54 D-Glyceraldehyde-3-phosphate
-
 Lactococcus lactis
2.3.1.54 D-Glyceraldehyde-3-phosphate
-
 Streptococcus equinus
2.3.1.54 dihydroxyacetone phosphate
-
 Lactococcus lactis
2.3.1.54 dihydroxyacetone phosphate
-
 Streptococcus equinus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.97.1.4 Fe2+ required Streptococcus equinus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.97.1.4 29900
-
 1 * 29900, calculated, 1 * 30000, SDS-PAGE Streptococcus equinus
1.97.1.4 30000
-
 gel filtration Streptococcus equinus
2.3.1.54 83000
-
 1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE Streptococcus equinus
2.3.1.54 87000
-
 1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE Streptococcus equinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.54 pyruvate + CoA Escherichia coli
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Lactococcus lactis
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Streptococcus equinus
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Clostridium pasteurianum
-
 acetyl-CoA + formate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.97.1.4 Streptococcus equinus Q93UQ7
-
-
2.3.1.54 Clostridium pasteurianum
-
-
-
2.3.1.54 Escherichia coli
-
-
-
2.3.1.54 Lactococcus lactis
-
-
-
2.3.1.54 Streptococcus equinus
-
 wild-type JB1
-
2.3.1.54 Streptococcus equinus O66391 wild-type JB1 pfl, GenBank nucleotide sequence accession number
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.54 recombinant enzyme Streptococcus equinus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.54 pyruvate + CoA
-
 Escherichia coli acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Escherichia coli acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA
-
 Lactococcus lactis acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Lactococcus lactis acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA
-
 Streptococcus equinus acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Streptococcus equinus acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA
-
 Clostridium pasteurianum acetyl-CoA + formate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.97.1.4 monomer 1 * 29900, calculated, 1 * 30000, SDS-PAGE Streptococcus equinus
2.3.1.54 dimer
-
 Escherichia coli
2.3.1.54 dimer
-
 Clostridium pasteurianum
2.3.1.54 dimer 1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE Streptococcus equinus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.54 PFL
-
 Escherichia coli
2.3.1.54 PFL
-
 Lactococcus lactis
2.3.1.54 PFL
-
 Streptococcus equinus