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Literature summary extracted from

  • Asanuma, N.; Hino, T.
    Effects of pH and energy supply on activity and amount of pyruvate formate-lyase in Streptococcus bovis (2000), Appl. Environ. Microbiol., 66, 3773-3777.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.54 pfl gene cloned and expressed in Escherichia coli HB101 Streptococcus equinus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.54 ATP
-
 Clostridium butyricum
2.3.1.54 ATP
-
 Lactococcus lactis
2.3.1.54 ATP
-
 Streptococcus mutans
2.3.1.54 D-Glyceraldehyde-3-phosphate
-
 Lactococcus lactis
2.3.1.54 D-Glyceraldehyde-3-phosphate
-
 Streptococcus equinus
2.3.1.54 D-Glyceraldehyde-3-phosphate
-
 Streptococcus mutans
2.3.1.54 dihydroxyacetone phosphate
-
 Lactococcus lactis
2.3.1.54 dihydroxyacetone phosphate
-
 Streptococcus equinus
2.3.1.54 dihydroxyacetone phosphate
-
 Streptococcus mutans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.54 1.6
-
 pyruvate
-
 Clostridium butyricum
2.3.1.54 2
-
 pyruvate
-
 Streptococcus equinus
2.3.1.54 2.6
-
 pyruvate
-
 Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.54 83000
-
 1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE Streptococcus equinus
2.3.1.54 87000
-
 1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE Streptococcus equinus
2.3.1.54 180000
-
 TOF-MS flight mass spectrometry Streptococcus equinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.54 pyruvate + CoA Escherichia coli
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Lactococcus lactis
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Streptococcus equinus
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Clostridium butyricum
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Streptococcus mutans
-
 acetyl-CoA + formate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.54 Clostridium butyricum
-
-
-
2.3.1.54 Escherichia coli
-
 K12
-
2.3.1.54 Escherichia coli
-
 ATCC 10798
-
2.3.1.54 Lactococcus lactis
-
 formerly designated Streptococcus cremoris
-
2.3.1.54 Lactococcus lactis
-
 formerly designated Streptococcus lactis
-
2.3.1.54 Streptococcus equinus
-
 wild-type JB1
-
2.3.1.54 Streptococcus mutans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.54
-
 Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.54 pyruvate + CoA
-
 Escherichia coli acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Escherichia coli acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA
-
 Lactococcus lactis acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Lactococcus lactis acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA
-
 Streptococcus equinus acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Streptococcus equinus acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA
-
 Clostridium butyricum acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Clostridium butyricum acetyl-CoA + formate
-
 r
2.3.1.54 pyruvate + CoA
-
 Streptococcus mutans acetyl-CoA + formate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.54 dimer
-
 Escherichia coli
2.3.1.54 dimer
-
 Streptococcus mutans
2.3.1.54 dimer 2 * 80000-90000, SDS-PAGE Streptococcus equinus
2.3.1.54 dimer 1 * 87000 + 1 * 83000, TOF-MS flight mass spectrometry, SDS-PAGE Streptococcus equinus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.54 PFL
-
 Escherichia coli
2.3.1.54 PFL
-
 Lactococcus lactis
2.3.1.54 PFL
-
 Streptococcus equinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.54 7.5
-
-
 Streptococcus equinus
2.3.1.54 7.5
-
-
 Clostridium butyricum
2.3.1.54 7.5
-
-
 Streptococcus mutans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.3.1.54 6
-
 activity decreases to less than 10% of the maximal activity Streptococcus equinus