Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Takahashi, S.; Abbe, K.; Yamada, T.
    Purification of pyruvate formate-lyase from Streptococcus mutans and its regulatory properties (1982), J. Bacteriol., 149, 1034-1040.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.54 Ferredoxin
-
 Escherichia coli
2.3.1.54 Ferredoxin
-
 Clostridium butyricum
2.3.1.54 Ferredoxin methylviologen can replace ferredoxin Streptococcus mutans
2.3.1.54 oxamate
-
 Escherichia coli
2.3.1.54 oxamate
-
 Streptococcus mutans
2.3.1.54 S-adenosylmethionine
-
 Escherichia coli
2.3.1.54 S-adenosylmethionine
-
 Clostridium butyricum
2.3.1.54 S-adenosylmethionine
-
 Streptococcus mutans

General Stability

EC Number General Stability Organism
2.3.1.54 unstable even when stored anaerobically Streptococcus mutans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.54 D-Glyceraldehyde-3-phosphate
-
 Lactococcus lactis
2.3.1.54 D-Glyceraldehyde-3-phosphate
-
 Streptococcus mutans
2.3.1.54 dihydroxyacetone phosphate
-
 Lactococcus lactis
2.3.1.54 dihydroxyacetone phosphate
-
 Streptococcus mutans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.54 90000
-
 2 * 90000, SDS-PAGE Streptococcus mutans
2.3.1.54 170000 180000 gel filtration Streptococcus mutans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.54 pyruvate + CoA Escherichia coli
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Lactococcus lactis
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Enterococcus faecalis
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Lactobacillus delbrueckii subsp. bulgaricus
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Clostridium butyricum
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Clostridium sp.
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Streptococcus mutans
-
 acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA Streptococcus mutans JC2
-
 acetyl-CoA + formate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.54 Clostridium butyricum
-
-
-
2.3.1.54 Clostridium sp.
-
-
-
2.3.1.54 Enterococcus faecalis
-
 previously Streptococcus faecalis
-
2.3.1.54 Escherichia coli
-
-
-
2.3.1.54 Lactobacillus delbrueckii subsp. bulgaricus
-
-
-
2.3.1.54 Lactococcus lactis
-
 formerly designated Streptococcus lactis
-
2.3.1.54 Streptococcus mutans
-
 JC2
-
2.3.1.54 Streptococcus mutans JC2
-
 JC2
-

Oxidation Stability

EC Number Oxidation Stability Organism
2.3.1.54 immediately inactivated by exposure to the air Streptococcus mutans

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.54
-
 Escherichia coli
2.3.1.54
-
 Enterococcus faecalis
2.3.1.54
-
 Streptococcus mutans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.54 12
-
-
 Streptococcus mutans

Storage Stability

EC Number Storage Stability Organism
2.3.1.54 4°C, 50 mM potassium phosphate buffer, containing 1 mM dithiothreitol, storage of the purified enzyme in an anaerobic glove box for 2 weeks reduces activity by 50% Streptococcus mutans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.54 pyruvate + CoA
-
 Escherichia coli acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Lactococcus lactis acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Enterococcus faecalis acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Lactobacillus delbrueckii subsp. bulgaricus acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Clostridium butyricum acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Clostridium sp. acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Streptococcus mutans acetyl-CoA + formate
-
 ?
2.3.1.54 pyruvate + CoA
-
 Streptococcus mutans JC2 acetyl-CoA + formate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.54 dimer
-
 Escherichia coli
2.3.1.54 dimer 2 * 90000, SDS-PAGE Streptococcus mutans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.54 22 35 activity is about 78 times of that at 22°C Streptococcus mutans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.54 7.5
-
-
 Clostridium butyricum
2.3.1.54 7.5
-
-
 Streptococcus mutans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.54 6.5 8.3 40% of activity maximum at pH 6.5, between pH 7.0-8.3 80% of maximum activity Streptococcus mutans