EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.51 | isolation of a cDNA encoding an enzyme by functional complementation of the Escherichia coli mutant plsC with an immature embryo cDNA library of oilseed rape. Transformation of the acyltransferase-deficient Escherichia coli strain JC201 with the cDNA sequence BAT2 alleviates the temperature-sensitive phenotype of the plsC mutant and confers a palmitoyl-CoA-preferring enzyme activity to membrane fractions. Mapping of the BAT2 genes and sequence analysis of the BAT2 cDNA clone | Brassica napus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.51 | chloroplast | mature protein of 32000 Da processed from the precursor protein of 38000 Da | Brassica napus | 9507 | - |
2.3.1.51 | membrane | - |
Brassica napus | 16020 | - |
2.3.1.51 | plastid | - |
Brassica napus | 9536 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.51 | 32000 | - |
mature protein, processing of a BAT2 precursor protein, chloroplast-import experiments | Brassica napus |
2.3.1.51 | 38000 | - |
predicted, protein encoded by the BAT2 cDNA | Brassica napus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.51 | additional information | Brassica napus | the enzyme controls phosphatidic acid biosynthesis in the plastid | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.51 | Brassica napus | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.51 | additional information | the BAT2 gene is expressed in a ubiquitous manner in all tissues, including nonphotosynthetic tissues where plastids are present | Brassica napus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.51 | acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | - |
Brassica napus | CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.51 | additional information | the enzyme controls phosphatidic acid biosynthesis in the plastid | Brassica napus | ? | - |
? | |
2.3.1.51 | oleoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | assayed with BAT2 protein | Brassica napus | CoA + 1,2-dioleoyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.51 | palmitoyl-ACP + 1-acyl-sn-glycerol 3-phosphate | - |
Brassica napus | ACP + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.51 | palmitoyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | assayed with BAT2 protein, palmitoyl-CoA is the preferred substrate | Brassica napus | CoA + 1-oleoyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.51 | 30 | - |
assay at | Brassica napus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.51 | 8 | - |
assay at | Brassica napus |