EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.51 | CHAPS | above 0.4% w/v | Pisum sativum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.51 | endoplasmic reticulum | - |
Pisum sativum | 5783 | - |
2.3.1.51 | microsome | predominantly located in endoplasmic reticulum | Pisum sativum | - |
- |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.51 | acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | Pisum sativum | involved in phospholipid biosynthesis | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.51 | additional information | Pisum sativum | acyl-CoA thioesters can be considered as the physiological substrates of the microsomal enzyme | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.51 | Pisum sativum | - |
pea, cv. Kleine Rheinlaenderin | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.51 | partial | Pisum sativum |
2.3.1.51 | using solubilization with CHAPS at high ionic strength, and anion-exchange chromatography on Mono Q column | Pisum sativum |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.51 | shoot | etiolated | Pisum sativum | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.51 | 0.0066 | - |
- |
Pisum sativum |
EC Number | Storage Stability | Organism |
---|---|---|
2.3.1.51 | -20°C, particulate preparation, several days | Pisum sativum |
2.3.1.51 | 4°C, soluble preparation, at least 1 day | Pisum sativum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.51 | acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | i.e. lysophosphatidic acid, highly specific for 1-acyl-sn-glycerol 3-phosphate, catalyzes acylation of position 2 hydroxyl-group | Pisum sativum | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? | |
2.3.1.51 | acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | unsaturated preferred | Pisum sativum | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? | |
2.3.1.51 | acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | pronounced specificities and selectivities for unsaturated C18-CoA thioesters | Pisum sativum | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | i.e. phosphatidic acid | ? | |
2.3.1.51 | acyl-CoA + 1-acyl-sn-glycerol 3-phosphate | involved in phospholipid biosynthesis | Pisum sativum | CoA + 1,2-diacyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.51 | acyl-CoA + 1-oleoyl-sn-glycerol 3-phosphate | 1-oleoyl-sn-glycerol 3-phosphate is the best acceptor | Pisum sativum | CoA + 2-acyl-1-oleoyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.51 | acyl-CoA + 1-palmitoyl-sn-glycerol 3-phosphate | - |
Pisum sativum | CoA + 1-palmitoyl-2-acyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.51 | acyl-CoA + 2-acyl-sn-glycerol 3-phosphate | the enzyme does not acylate 2-acyl-sn-glycerol 3-phosphate | Pisum sativum | CoA + ? | - |
? | |
2.3.1.51 | acyl-CoA + linoleoyl-sn-glycerol 3-phosphate | - |
Pisum sativum | CoA + ? | - |
? | |
2.3.1.51 | acyl-CoA + myristoyl-sn-glycerol 3-phosphate | - |
Pisum sativum | CoA + ? | - |
? | |
2.3.1.51 | gondoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | gondoyl-CoA is utilized less efficiently for the reaction than oleoyl-CoA | Pisum sativum | CoA + 1-acyl-2-gondoyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.51 | additional information | the microsomal enzyme displays a specificity for unsaturated C18-CoA thioesters and this fatty-acid specificity is not altered in the course of the purification procedure | Pisum sativum | ? | - |
? | |
2.3.1.51 | additional information | acyl-CoA thioesters can be considered as the physiological substrates of the microsomal enzyme | Pisum sativum | ? | - |
? | |
2.3.1.51 | oleoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | oleoyl-CoA is the best substrate, both membrane-bound and partially purified enzyme | Pisum sativum | CoA + 1-acyl-2-oleoyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.51 | palmitoyl-ACP + 1-acyl-sn-glycerol 3-phosphate | - |
Pisum sativum | ACP + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? | |
2.3.1.51 | palmitoyl-CoA + 1-acyl-sn-glycerol 3-phosphate | - |
Pisum sativum | CoA + 1-acyl-2-palmitoyl-sn-glycerol 3-phosphate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.51 | 25 | - |
assay at | Pisum sativum |