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Literature summary extracted from

  • Eberharter, A.; Lechner, T.; Goralik-Schramel, M.; Loidl, P.
    Purification and characterization of the cytoplasmic histone acetyltransferase B of maize embryos (1996), FEBS Lett., 386, 75-81.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.48 Brij-58 no inhibition Zea mays
2.3.1.48 Cu2+ 5 mM, enzyme form B Zea mays
2.3.1.48 dimethyl sulfoxide irreversible at 2% v/v Zea mays
2.3.1.48 ethanol irreversible at 2% v/v Zea mays
2.3.1.48 Fe2+ 5 mM, enzyme form B Zea mays
2.3.1.48 Isopropanol irreversible at 2% v/v Zea mays
2.3.1.48 Triton X-100 no inhibition Zea mays
2.3.1.48 Zn2+ 5 mM, enzyme form B Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.48 cytoplasm enzyme form B Zea mays 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.48 45000
-
 1 * 45000 + 1 * 50000, enzyme form B, SDS-PAGE Zea mays
2.3.1.48 50000
-
 1 * 45000 + 1 * 50000, enzyme form B, SDS-PAGE Zea mays
2.3.1.48 90000
-
 enzyme form B, gel filtration Zea mays

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.3.1.48 dimethyl sulfoxide irreversible inactivation at 2% v/v Zea mays
2.3.1.48 Ethanol irreversible inactivation at 2% v/v Zea mays
2.3.1.48 isopropanol irreversible inactivation at 2% v/v Zea mays

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.48 Zea mays
-
-
-
2.3.1.48 Zea mays
-
 enzyme form B
-
2.3.1.48 Zea mays Cuzco
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.48 enzyme form B Zea mays

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.48 seedling i.e. embryo Zea mays
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.48 additional information
-
-
 Zea mays
2.3.1.48 0.0057
-
 purified enzyme form B Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.48 2 acetyl-CoA + histone H4 enzyme form B Zea mays 2 CoA + diacetylhistone H4 mono- and diacetylated products ?
2.3.1.48 2 acetyl-CoA + histone H4 enzyme form B Zea mays Cuzco 2 CoA + diacetylhistone H4 mono- and diacetylated products ?
2.3.1.48 acetyl-CoA + histone
-
 Zea mays CoA + acetylhistone
-
 ?
2.3.1.48 acetyl-CoA + histone
-
 Zea mays Cuzco CoA + acetylhistone
-
 ?
2.3.1.48 acetyl-CoA + histone H3 enzyme form B, very low activity Zea mays CoA + acetylhistone H3
-
 ?
2.3.1.48 acetyl-CoA + histone H3 enzyme form B, very low activity Zea mays Cuzco CoA + acetylhistone H3
-
 ?
2.3.1.48 acetyl-CoA + histone H4 enzyme form B Zea mays CoA + acetylhistone H4 mono- and diacetylated products ?
2.3.1.48 acetyl-CoA + histone H4 enzyme form B Zea mays Cuzco CoA + acetylhistone H4 mono- and diacetylated products ?

Subunits

EC Number Subunits Comment Organism
2.3.1.48 dimer 1 * 45000 + 1 * 50000, enzyme form B, SDS-PAGE Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.48 37
-
 enzyme form B Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.48 additional information
-
 enzyme is highly unstable during purification, solubilization at -10°C Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.48 8.2 8.5 enzyme form B Zea mays