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Literature summary extracted from

  • Webster, S.P.; Campopiano, D.J.; Alexeev, D.; Alexeeva, M.; Watt, R.M.; Sawyer, L.; Baxter, R.L.
    Characterization of 8-amino-7-oxononanoate synthase: a bacterial PLP-dependent, acyl CoA condensing enzyme (1998), Biochem. Soc. Trans., 26, S268.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.47 bioF gene, expression in strain HMS174 (DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.47 pH 8.5 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.47 0.07
-
 pimeloyl-CoA
-
 Escherichia coli
2.3.1.47 0.9
-
 L-alanine
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.47 41000
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Escherichia coli i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Escherichia coli reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.47 Escherichia coli
-
 gene bioF
-
2.3.1.47 Lysinibacillus sphaericus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.47 recombinant from overexpressing E. coli strain HMS174 (DE3) Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.47 pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] reaction mechanism Escherichia coli
a
2.3.1.47 pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] reaction mechanism Lysinibacillus sphaericus
a
2.3.1.47 pimeloyl-[acyl-carrier protein] + L-alanine = 8-amino-7-oxononanoate + CO2 + holo-[acyl-carrier protein] reaction mechanism, formation of the pyridoxal-phosphate-stabilized carbanion by abstraction of the C2-H proton of the alanine-pyridoxal-phosphate aldimine, followed by acylation and decarboxylation Lysinibacillus sphaericus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Escherichia coli 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Escherichia coli 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ?

Subunits

EC Number Subunits Comment Organism
2.3.1.47 More homodimer with each monomer consisting of 3 domains Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.3.1.47 AONS
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.47 0.000833
-
 pimeloyl-CoA
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.47 pyridoxal 5'-phosphate
-
 Escherichia coli
2.3.1.47 pyridoxal 5'-phosphate
-
 Lysinibacillus sphaericus