Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ploux, O.; Marquet, A.
    The 8-amino-7-oxopelargonate synthase from Bacillus sphaericus. Purification and preliminary characterization of the cloned enzyme overproduced in Escherichia coli (1992), Biochem. J., 283, 327-331.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.47 expressed in overproducing Escherichia coli strain C268/pTG3401 Lysinibacillus sphaericus

General Stability

EC Number General Stability Organism
2.3.1.47 EDTA stabilizes during purification Lysinibacillus sphaericus
2.3.1.47 freeze-thawing inactivates Lysinibacillus sphaericus
2.3.1.47 proteinase inhibitors, e.g. PMSF, pepstatin A, stabilize during purification Lysinibacillus sphaericus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.47 0.001
-
 pimeloyl-CoA
-
 Lysinibacillus sphaericus
2.3.1.47 3
-
 L-alanine
-
 Lysinibacillus sphaericus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.47 additional information
-
 amino acid composition Lysinibacillus sphaericus
2.3.1.47 41000
-
 1 * 41000, SDS-PAGE Lysinibacillus sphaericus
2.3.1.47 46000
-
 gel filtration Lysinibacillus sphaericus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus i.e. pimeloyl-CoA 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine Lysinibacillus sphaericus reaction in biotin biosynthesis 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.47 Lysinibacillus sphaericus
-
 gene bioF
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.47 recombinant from E. coli overproducing strain Lysinibacillus sphaericus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.47 0.67
-
-
 Lysinibacillus sphaericus

Storage Stability

EC Number Storage Stability Organism
2.3.1.47 0°C, several months Lysinibacillus sphaericus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine i.e. pimeloyl-CoA Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir
2.3.1.47 6-carboxyhexanoyl-CoA + L-alanine reaction in biotin biosynthesis Lysinibacillus sphaericus 8-amino-7-oxononanoate + CoA + CO2 i.e. 7-oxo-8-amino-pelargonic acid ir

Subunits

EC Number Subunits Comment Organism
2.3.1.47 monomer 1 * 41000, SDS-PAGE Lysinibacillus sphaericus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.47 AOP synthase
-
 Lysinibacillus sphaericus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.47 37
-
 assay at Lysinibacillus sphaericus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.47 0.5
-
 pimeloyl-CoA
-
 Lysinibacillus sphaericus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.47 additional information
-
 pI: 5.2 Lysinibacillus sphaericus
2.3.1.47 7
-
 assay at Lysinibacillus sphaericus

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.47 pyridoxal 5'-phosphate dependent on Lysinibacillus sphaericus