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Literature summary extracted from

  • Corfield, A.P.; Ferreira do Amarral, C.; Wember, M.; Schauer, R.
    The metabolism of O-acyl-N-acylneuraminic acids. Biosynthesis of O-acylated sialic acids in bovine and equine submandibular glands (1976), Eur. J. Biochem., 68, 597-610.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.45 lyophilisation causes loss of 20-30% activity, storage in lyophilized stage leads to additional loss of 60% activity Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.45 CoA inhibition of membrane-bound enzyme Bos taurus
2.3.1.45 iodoacetate
-
 Bos taurus
2.3.1.45 additional information no inhibition by MgCl2, CaCl2, MnCl2 Bos taurus
2.3.1.45 p-chloromercuribenzoate
-
 Bos taurus
2.3.1.45 Triton X-100 no inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.45 0.01
-
 acetyl-CoA cytosolic enzyme Bos taurus
2.3.1.45 0.12
-
 acetyl-CoA membrane-bound enzyme Bos taurus
2.3.1.45 0.39
-
 N-glycolylneuraminate cytosolic enzyme Bos taurus
2.3.1.45 0.5
-
 N-acetylneuraminate cytosolic enzyme Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.45 microsome
-
 Bos taurus
-
-
2.3.1.45 microsome
-
 Equus caballus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.45 acetyl-CoA + sialic acid Bos taurus
-
 CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
 ?
2.3.1.45 acetyl-CoA + sialic acid Equus caballus
-
 CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.45 Bos taurus
-
-
-
2.3.1.45 Equus caballus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.45 submandibular gland
-
 Bos taurus
-
2.3.1.45 submandibular gland
-
 Equus caballus
-

Storage Stability

EC Number Storage Stability Organism
2.3.1.45 0°C, 16 days, 55% loss of activity, membrane-bound enzyme Bos taurus
2.3.1.45 frozen enzyme is stable for at least 6 months Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.45 acetyl-CoA + N-acetylneuraminate
-
 Equus caballus CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
 ?
2.3.1.45 acetyl-CoA + N-acetylneuraminate the membrane-bound enzyme acts on endogenous, glycoprotein-bound N-acetyl- and N-glycolylneuraminic acids, the soluble enzyme acts on exogenous non-glycosidically bound N-acetyl- and N-glycolylneuraminic acids Bos taurus CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
 ?
2.3.1.45 acetyl-CoA + N-glycolylneuraminate
-
 Bos taurus ?
-
 ?
2.3.1.45 acetyl-CoA + sialic acid
-
 Bos taurus CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
 ?
2.3.1.45 acetyl-CoA + sialic acid
-
 Equus caballus CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.45 37
-
-
 Bos taurus
2.3.1.45 37
-
-
 Equus caballus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.45 7
-
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.45 0.56
-
 CoA
-
 Bos taurus