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Literature summary extracted from

  • Schauer, R.
    Biosynthesis of N-acetyl-O-acetylneuraminic acids. I. Incorporation of (14C) acetate into sections of the submaxillary salivary gland of ox and horse (1970), Hoppe-Seyler's Z. Physiol. Chem., 351, 595-602.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.45 activity is stable to the first cycle of freezing, cannot be refrozen after thawing Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.45 diethyl dicarbonate
-
Bos taurus
2.3.1.45 N-bromosuccinimide
-
Bos taurus
2.3.1.45 Triton X-100 above 0.02% Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.44 acetyl-CoA + N-acetylneuraminate Equus caballus
-
CoA + N-acetyl-4-O-acetylneuraminate
-
?
2.3.1.45 acetyl-CoA + sialic acid Bos taurus
-
CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.44 Equus caballus
-
-
-
2.3.1.45 Bos taurus
-
ox
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.44 submaxillary gland
-
Equus caballus
-
2.3.1.45 submaxillary gland
-
Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.44 acetyl-CoA + N-acetylneuraminate
-
Equus caballus CoA + N-acetyl-4-O-acetylneuraminate
-
?
2.3.1.45 acetyl-CoA + N-acetylneuraminate
-
Bos taurus CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
?
2.3.1.45 acetyl-CoA + sialic acid
-
Bos taurus CoA + N-acetyl-7-O(or 9-O-)-acetylneuraminate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.45 More in early days of investigation of sialate O-acetyltransferase it was not possible to discriminate clearly between 7- or 9-O-acetylated sialic acids enzymatically formed on a micro scale in order to denominate the enzyme correctly, there also exists the possibility of the existence of more than one O-acetyltransferases incorporating acetyl-groups into different positions of the sialic acid side chain, in contrast a hypothesis is presented for the requirement of only one transferase specific for the 7-OH group, after insertion of the O-acetyl group at O-7 the ester residue may migrate to O-9 afterwards a second O-acetyl group can be transferred by the same enzyme to O-7, which may migrate to O-8 and open the way for linking a third acetyl group to O-7 Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.45 22
-
50% loss of activity after 20 min at room temperature Bos taurus
2.3.1.45 37
-
activity is very labile Bos taurus