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Literature summary extracted from

  • Chisholm, J.W.; Gebre, A.K.; Parks, J.S.
    Characterization of C-terminal histidine-tagged human recombinant lecithin:cholesterol acyltransferase (1999), J. Lipid Res., 40, 1512-1519.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.43 expression of a C-terminal histidine tagged enzyme in Chinese hamster ovary cells Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.43 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.43 glycoprotein a recombinant C-terminal histidine tagged enzyme has similar carbohydrate moieties than the enzyme purified from plasma Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.43 purification of a recombinant C-terminal histidine tagged enzyme using a cobalt affinity resin Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.43 0.43
-
 recombinant C-terminal histidine tagged enzyme, similar to plasma enzyme Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.3.1.43 4°C, 60 days Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.43 cholesterol + 1-palmitoyl-2-oleoylphosphatidylcholine
-
 Homo sapiens cholesteryl oleate + 1-palmitoylglycerophosphocholine
-
 ?
2.3.1.43 phosphatidylcholine + sterol
-
 Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
 ?