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Literature summary extracted from

  • Chen, C.H.; Albers, J.J.
    A rapid large-scale procedure for purification of lecithin-cholesterol acyltransferase from human and animal plasma (1985), Biochim. Biophys. Acta, 834, 188-195.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.43 apolipoprotein A-I
-
Homo sapiens
2.3.1.43 apolipoprotein A-I
-
Sus scrofa
2.3.1.43 apolipoprotein A-I
-
Oryctolagus cuniculus
2.3.1.43 apolipoprotein A-I
-
Canis lupus familiaris
2.3.1.43 apolipoprotein A-I
-
Capra hircus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.43 p-hydroxymercuribenzoate
-
Canis lupus familiaris
2.3.1.43 p-hydroxymercuribenzoate
-
Capra hircus
2.3.1.43 p-hydroxymercuribenzoate
-
Homo sapiens
2.3.1.43 p-hydroxymercuribenzoate
-
Oryctolagus cuniculus
2.3.1.43 p-hydroxymercuribenzoate
-
Sus scrofa
2.3.1.43 phenylmethylsulfonyl fluoride
-
Canis lupus familiaris
2.3.1.43 phenylmethylsulfonyl fluoride
-
Capra hircus
2.3.1.43 phenylmethylsulfonyl fluoride
-
Homo sapiens
2.3.1.43 phenylmethylsulfonyl fluoride
-
Oryctolagus cuniculus
2.3.1.43 phenylmethylsulfonyl fluoride
-
Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.43 65000
-
x * 65000, SDS-PAGE Homo sapiens
2.3.1.43 65000
-
x * 65000, SDS-PAGE Canis lupus familiaris
2.3.1.43 65000
-
x * 65000, SDS-PAGE Capra hircus
2.3.1.43 66000
-
-
Sus scrofa
2.3.1.43 66000
-
HPLC gel filtration Canis lupus familiaris
2.3.1.43 66000
-
HPLC gel filtration Capra hircus
2.3.1.43 66000
-
x * 66000, SDS-PAGE Oryctolagus cuniculus
2.3.1.43 67000
-
HPLC gel filtration Homo sapiens
2.3.1.43 67000
-
HPLC gel filtration Sus scrofa
2.3.1.43 67000
-
HPLC gel filtration Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.43 Canis lupus familiaris
-
dog
-
2.3.1.43 Capra hircus
-
goat
-
2.3.1.43 Homo sapiens
-
-
-
2.3.1.43 Oryctolagus cuniculus
-
rabbit
-
2.3.1.43 Sus scrofa
-
pig
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.43 precipitation from plasma with dextran sulfate-Mg2+ followed by several chromatographic steps, including DEAE-Sepharose, phenyl-Sepharose and affinity chromatography Homo sapiens
2.3.1.43 precipitation from plasma with dextran sulfate-Mg2+ followed by several chromatographic steps, including DEAE-Sepharose, phenyl-Sepharose and affinity chromatography Sus scrofa
2.3.1.43 precipitation from plasma with dextran sulfate-Mg2+ followed by several chromatographic steps, including DEAE-Sepharose, phenyl-Sepharose and affinity chromatography Oryctolagus cuniculus
2.3.1.43 precipitation from plasma with dextran sulfate-Mg2+ followed by several chromatographic steps, including DEAE-Sepharose, phenyl-Sepharose and affinity chromatography Canis lupus familiaris
2.3.1.43 precipitation from plasma with dextran sulfate-Mg2+ followed by several chromatographic steps, including DEAE-Sepharose, phenyl-Sepharose and affinity chromatography Capra hircus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.43 plasma
-
Homo sapiens
-
2.3.1.43 plasma
-
Sus scrofa
-
2.3.1.43 plasma
-
Oryctolagus cuniculus
-
2.3.1.43 plasma
-
Canis lupus familiaris
-
2.3.1.43 plasma
-
Capra hircus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.43 0.55
-
in cholesterol-lecithin vesicles activated with human apolipoprotein A1 Capra hircus
2.3.1.43 0.56
-
in cholesterol-lecithin vesicles activated with human apolipoprotein A1 Oryctolagus cuniculus
2.3.1.43 0.57
-
in cholesterol-lecithin vesicles activated with apolipoprotein A1 Homo sapiens
2.3.1.43 0.57
-
in cholesterol-lecithin vesicles activated with apolipoprotein A1 Canis lupus familiaris
2.3.1.43 0.59
-
in cholesterol-lecithin vesicles activated with human apolipoprotein A1 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.43 phosphatidylcholine + sterol
-
Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
r
2.3.1.43 phosphatidylcholine + sterol
-
Sus scrofa 1-acylglycerophosphocholine + sterol ester
-
r
2.3.1.43 phosphatidylcholine + sterol
-
Oryctolagus cuniculus 1-acylglycerophosphocholine + sterol ester
-
r
2.3.1.43 phosphatidylcholine + sterol
-
Canis lupus familiaris 1-acylglycerophosphocholine + sterol ester
-
r
2.3.1.43 phosphatidylcholine + sterol
-
Capra hircus 1-acylglycerophosphocholine + sterol ester
-
r

Subunits

EC Number Subunits Comment Organism
2.3.1.43 ? x * 66000, SDS-PAGE Sus scrofa
2.3.1.43 ? x * 66000, SDS-PAGE Oryctolagus cuniculus
2.3.1.43 ? x * 65000, SDS-PAGE Homo sapiens
2.3.1.43 ? x * 65000, SDS-PAGE Canis lupus familiaris
2.3.1.43 ? x * 65000, SDS-PAGE Capra hircus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.43 7.6
-
-
Oryctolagus cuniculus
2.3.1.43 7.6
-
-
Canis lupus familiaris
2.3.1.43 7.8
-
-
Sus scrofa
2.3.1.43 8
-
-
Homo sapiens
2.3.1.43 8
-
-
Capra hircus