Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Doi, Y.; Nishida, T.
    Lecithin-cholesterol acyltransferase from human plasma (1981), Methods Enzymol., 71, 753-767.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.43 apolipoprotein A-I absolute requirement with phosphatidylcholine-cholesterol vesicles as a substrate Homo sapiens
2.3.1.43 Apolipoprotein C-1 cofactor activity, less effective than apo-A-1 Homo sapiens
2.3.1.43 additional information apo-A-II, apo-C-III and apo-D inhibit activation of enzyme by apo-A-1 Homo sapiens

General Stability

EC Number General Stability Organism
2.3.1.43 apo-A-1, albumin and lecithin-cholesterol vesicles stabilize Homo sapiens
2.3.1.43 glycerol stabilizes during storage at -20°C Homo sapiens
2.3.1.43 lability to freezing and thawing and to high ionic strength Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.43 2-mercaptoethanol at high concentration Homo sapiens
2.3.1.43 dithiothreitol at high concentration Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.43 59000
-
 sedimentation equilibrium ultracentrifugation Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.43 phosphatidylcholine + sterol Homo sapiens important in metabolism and structure of plasma lipoproteins 1-acylglycerophosphocholine + sterol ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.43 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.43 glycoprotein 24% carbohydrate by weight, 13 mol of mannose, 30 mol of galactose, 17 mol of glucosamine and 13 mol of sialic acid per mol of enzyme protein Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.43 plasma
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.43 2.7
-
 in cholesterol-lecithin vesicles Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.43 additional information long-chain primary alcohols can act as acyl acceptors Homo sapiens ?
-
 ?
2.3.1.43 additional information lecithin-cholesterol single bilayer vesicles as substrate Homo sapiens ?
-
 ?
2.3.1.43 phosphatidylcholine + sterol wide specificity for acyl acceptor: sterols with a beta-configuration at carbon-3 and trans fused A /B rings Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
 r
2.3.1.43 phosphatidylcholine + sterol cholesterol as substrate Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
 r
2.3.1.43 phosphatidylcholine + sterol acyl donor: high degree of specificity for phospholipids containing a basic nitrogen atom Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
 r
2.3.1.43 phosphatidylcholine + sterol important in metabolism and structure of plasma lipoproteins Homo sapiens 1-acylglycerophosphocholine + sterol ester
-
 ?