Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Webber, K.O.; Hajra, A.K.
    Dihydroxyacetone phosphate acyltransferase (1992), Methods Enzymol., 209, 92-98.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.42 asolectin activation, i.e. crude soybean phospholipid preparation containing phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol Cavia porcellus
2.3.1.42 bovine serum albumin activation, by inhibiting the formation of palmitoyl-CoA micelles Cavia porcellus
2.3.1.42 additional information heating membranes to 50°C prior to assay increases activity of membrane-bound enzyme Cavia porcellus
2.3.1.42 Phospholipid
-
 Cavia porcellus

General Stability

EC Number General Stability Organism
2.3.1.42 heating membranes to 50°C prior to assay increases activity of membrane-bound enzyme Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.42 5,5'-dithio-bis(2-nitrobenzoate) 5 mM, moderate; i.e. DTNB Cavia porcellus
2.3.1.42 iodoacetamide not Cavia porcellus
2.3.1.42 N-ethylmaleimide not peroxisomal enzyme Cavia porcellus
2.3.1.42 p-chloromercuriphenylsulfonic acid moderate Cavia porcellus
2.3.1.42 palmitoyl-CoA bovine serum albumin protects Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.42 additional information
-
 additional information
-
 Cavia porcellus
2.3.1.42 0.04 0.2 dihydroxyacetone phosphate membrane-bound enzyme, pH 7.4 Cavia porcellus
2.3.1.42 0.06
-
 dihydroxyacetone phosphate purified enzyme, pH 7.4 Cavia porcellus
2.3.1.42 0.1 0.5 dihydroxyacetone phosphate solubilized, pH 7.4 Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.42 peroxisome
-
 Cavia porcellus 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.42 69000
-
 x * 69000, SDS-PAGE Cavia porcellus
2.3.1.42 90000
-
 gel filtration Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.42 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.42
-
 Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.42 liver
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.42 3.35
-
 purified enzyme Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.42 acyl-CoA + dihydroxyacetone phosphate
-
 Cavia porcellus CoA + acyldihydroxyacetone phosphate i.e. acylglycerone phosphate ?
2.3.1.42 palmitoyl-CoA + dihydroxyacetone phosphate
-
 Cavia porcellus CoA + monopalmitoyldihydroxyacetone phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.42 ? x * 69000, SDS-PAGE Cavia porcellus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.42 37
-
 assay at Cavia porcellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.42 additional information
-
 membrane-bound: heat-stable Cavia porcellus
2.3.1.42 additional information
-
 solubilized: heat-labile Cavia porcellus
2.3.1.42 40
-
 significant loss of activity Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.42 5.5
-
 membrane-bound peroxisomal enzyme Cavia porcellus
2.3.1.42 7.4
-
 detergent-solubilized enzyme Cavia porcellus