BRENDA - Enzyme Database

Solubilization and partial purification of dihydroxyacetone-phosphate acyltransferase from guinea pig liver

Jones, C.L.; Hajra, A.K.; Arch. Biochem. Biophys. 226, 155-165 (1983)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.42
asolectin
activation, i.e. crude soybean phospholipid preparation containing phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol
Cavia porcellus
2.3.1.42
Phospholipid
activation, e.g. phosphatidylcholine, dilauroylphosphatidylcholine; at low concentration: phosphatidylserine and phosphatidylethanolamine, both inhibitory at high concentration; crude peroxisomal phospholipids; phosphatidylglycerol
Cavia porcellus
General Stability
EC Number
General Stability
Organism
2.3.1.42
asolectin stabilizes against inactivation during freezing and thawing
Cavia porcellus
2.3.1.42
DEAE-Sephacel chromatography inactivates
Cavia porcellus
2.3.1.42
freezing inactivates
Cavia porcellus
2.3.1.42
isoelectric focusing inactivates
Cavia porcellus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.42
5,5'-dithio-bis(2-nitrobenzoate)
i.e. DTNB
Cavia porcellus
2.3.1.42
Cd2+
-
Cavia porcellus
2.3.1.42
cholate
above 2 mg/ml
Cavia porcellus
2.3.1.42
Cu2+
-
Cavia porcellus
2.3.1.42
iodoacetamide
5 mM, weak
Cavia porcellus
2.3.1.42
p-chloromercuriphenylsulfonic acid
5 mM
Cavia porcellus
2.3.1.42
phosphatidic acid
-
Cavia porcellus
2.3.1.42
phosphatidylethanolamine
above 1.5 mM
Cavia porcellus
2.3.1.42
phosphatidylinositol
-
Cavia porcellus
2.3.1.42
phosphatidylserine
above 1.5 mM
Cavia porcellus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.42
additional information
-
additional information
-
Cavia porcellus
2.3.1.42
0.065
-
palmitoyl-CoA
-
Cavia porcellus
2.3.1.42
0.1
0.5
dihydroxyacetone phosphate
in presence of asolectin
Cavia porcellus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.42
peroxisome
-
Cavia porcellus
5777
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.42
additional information
no activation by Ca2+; no activation by Mg2+, partially purified; no activation by Mn2+, partially purified
Cavia porcellus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.42
Cavia porcellus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.42
partial, solubilized with sodium cholate-KCl
Cavia porcellus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.42
liver
-
Cavia porcellus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.42
0.106
-
-
Cavia porcellus
2.3.1.42
0.212
-
-
Cavia porcellus
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.42
2-4C, 30-50% loss of activity after 1 month, DTT stabilizes during storage
Cavia porcellus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.42
acyl-CoA + dihydroxyacetone phosphate
i.e. glycerone phosphate, saturated acyl-CoAs preferred over unsaturated
486953
Cavia porcellus
CoA + acyldihydroxyacetone phosphate
i.e. acylglycerone phosphate
486953
Cavia porcellus
r
2.3.1.42
arachidonoyl-CoA + dihydroxyacetone phosphate
i.e. eicosatetraenoyl-CoA, poor substrate
486953
Cavia porcellus
CoA + arachidonoyldihydroxyacetone phosphate
-
-
-
r
2.3.1.42
linoleoyl-CoA + dihydroxyacetone phosphate
-
486953
Cavia porcellus
CoA + linoleoyldihydroxyacetone phosphate
-
-
-
r
2.3.1.42
oleoyl-CoA + dihydroxyacetone phosphate
poor substrate
486953
Cavia porcellus
CoA + oleoyldihydroxyacetone phosphate
-
-
-
r
2.3.1.42
palmitoyl-CoA + dihydroxyacetone phosphate
best substrate
486953
Cavia porcellus
CoA + monopalmitoyldihydroxyacetone phosphate
-
486953
Cavia porcellus
r
2.3.1.42
stearoyl-CoA + dihydroxyacetone phosphate
acylation at 35% the rate of palmitoyl-CoA
486953
Cavia porcellus
CoA + stearoyldihydroxyacetone phosphate
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.42
37
-
assay at
Cavia porcellus
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.3.1.42
48
-
t1/2: 15 min
Cavia porcellus
2.3.1.42
51
-
t1/2: 15 min, with asolectin
Cavia porcellus
2.3.1.42
60
-
10 min stable; intact peroxisomes
Cavia porcellus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.42
6.4
-
in presence of asolectin
Cavia porcellus
2.3.1.42
7
8.2
broad
Cavia porcellus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.42
6
9
about half-maximal activity at pH 6.0 and pH 9.0, in the presence of asolectin
Cavia porcellus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.3.1.42
asolectin
activation, i.e. crude soybean phospholipid preparation containing phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol
Cavia porcellus
2.3.1.42
Phospholipid
activation, e.g. phosphatidylcholine, dilauroylphosphatidylcholine; at low concentration: phosphatidylserine and phosphatidylethanolamine, both inhibitory at high concentration; crude peroxisomal phospholipids; phosphatidylglycerol
Cavia porcellus
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.42
asolectin stabilizes against inactivation during freezing and thawing
Cavia porcellus
2.3.1.42
DEAE-Sephacel chromatography inactivates
Cavia porcellus
2.3.1.42
freezing inactivates
Cavia porcellus
2.3.1.42
isoelectric focusing inactivates
Cavia porcellus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.42
5,5'-dithio-bis(2-nitrobenzoate)
i.e. DTNB
Cavia porcellus
2.3.1.42
Cd2+
-
Cavia porcellus
2.3.1.42
cholate
above 2 mg/ml
Cavia porcellus
2.3.1.42
Cu2+
-
Cavia porcellus
2.3.1.42
iodoacetamide
5 mM, weak
Cavia porcellus
2.3.1.42
p-chloromercuriphenylsulfonic acid
5 mM
Cavia porcellus
2.3.1.42
phosphatidic acid
-
Cavia porcellus
2.3.1.42
phosphatidylethanolamine
above 1.5 mM
Cavia porcellus
2.3.1.42
phosphatidylinositol
-
Cavia porcellus
2.3.1.42
phosphatidylserine
above 1.5 mM
Cavia porcellus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.42
additional information
-
additional information
-
Cavia porcellus
2.3.1.42
0.065
-
palmitoyl-CoA
-
Cavia porcellus
2.3.1.42
0.1
0.5
dihydroxyacetone phosphate
in presence of asolectin
Cavia porcellus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.3.1.42
peroxisome
-
Cavia porcellus
5777
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.3.1.42
additional information
no activation by Ca2+; no activation by Mg2+, partially purified; no activation by Mn2+, partially purified
Cavia porcellus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.42
partial, solubilized with sodium cholate-KCl
Cavia porcellus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.42
liver
-
Cavia porcellus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
2.3.1.42
0.106
-
-
Cavia porcellus
2.3.1.42
0.212
-
-
Cavia porcellus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.42
2-4C, 30-50% loss of activity after 1 month, DTT stabilizes during storage
Cavia porcellus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.42
acyl-CoA + dihydroxyacetone phosphate
i.e. glycerone phosphate, saturated acyl-CoAs preferred over unsaturated
486953
Cavia porcellus
CoA + acyldihydroxyacetone phosphate
i.e. acylglycerone phosphate
486953
Cavia porcellus
r
2.3.1.42
arachidonoyl-CoA + dihydroxyacetone phosphate
i.e. eicosatetraenoyl-CoA, poor substrate
486953
Cavia porcellus
CoA + arachidonoyldihydroxyacetone phosphate
-
-
-
r
2.3.1.42
linoleoyl-CoA + dihydroxyacetone phosphate
-
486953
Cavia porcellus
CoA + linoleoyldihydroxyacetone phosphate
-
-
-
r
2.3.1.42
oleoyl-CoA + dihydroxyacetone phosphate
poor substrate
486953
Cavia porcellus
CoA + oleoyldihydroxyacetone phosphate
-
-
-
r
2.3.1.42
palmitoyl-CoA + dihydroxyacetone phosphate
best substrate
486953
Cavia porcellus
CoA + monopalmitoyldihydroxyacetone phosphate
-
486953
Cavia porcellus
r
2.3.1.42
stearoyl-CoA + dihydroxyacetone phosphate
acylation at 35% the rate of palmitoyl-CoA
486953
Cavia porcellus
CoA + stearoyldihydroxyacetone phosphate
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
2.3.1.42
37
-
assay at
Cavia porcellus
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
2.3.1.42
48
-
t1/2: 15 min
Cavia porcellus
2.3.1.42
51
-
t1/2: 15 min, with asolectin
Cavia porcellus
2.3.1.42
60
-
10 min stable; intact peroxisomes
Cavia porcellus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.42
6.4
-
in presence of asolectin
Cavia porcellus
2.3.1.42
7
8.2
broad
Cavia porcellus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.42
6
9
about half-maximal activity at pH 6.0 and pH 9.0, in the presence of asolectin
Cavia porcellus