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Literature summary extracted from

  • Jones, C.L.; Hajra, A.K.
    Solubilization and partial purification of dihydroxyacetone-phosphate acyltransferase from guinea pig liver (1983), Arch. Biochem. Biophys., 226, 155-165.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.42 asolectin activation, i.e. crude soybean phospholipid preparation containing phosphatidylcholine, phosphatidylethanolamine and phosphatidylinositol Cavia porcellus
2.3.1.42 Phospholipid crude peroxisomal phospholipids Cavia porcellus
2.3.1.42 Phospholipid at low concentration: phosphatidylserine and phosphatidylethanolamine, both inhibitory at high concentration Cavia porcellus
2.3.1.42 Phospholipid phosphatidylglycerol Cavia porcellus
2.3.1.42 Phospholipid activation, e.g. phosphatidylcholine, dilauroylphosphatidylcholine Cavia porcellus

General Stability

EC Number General Stability Organism
2.3.1.42 asolectin stabilizes against inactivation during freezing and thawing Cavia porcellus
2.3.1.42 DEAE-Sephacel chromatography inactivates Cavia porcellus
2.3.1.42 freezing inactivates Cavia porcellus
2.3.1.42 isoelectric focusing inactivates Cavia porcellus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.42 5,5'-dithio-bis(2-nitrobenzoate) i.e. DTNB Cavia porcellus
2.3.1.42 Cd2+
-
 Cavia porcellus
2.3.1.42 cholate above 2 mg/ml Cavia porcellus
2.3.1.42 Cu2+
-
 Cavia porcellus
2.3.1.42 iodoacetamide 5 mM, weak Cavia porcellus
2.3.1.42 p-chloromercuriphenylsulfonic acid 5 mM Cavia porcellus
2.3.1.42 phosphatidic acid
-
 Cavia porcellus
2.3.1.42 phosphatidylethanolamine above 1.5 mM Cavia porcellus
2.3.1.42 phosphatidylinositol
-
 Cavia porcellus
2.3.1.42 phosphatidylserine above 1.5 mM Cavia porcellus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.42 additional information
-
 additional information
-
 Cavia porcellus
2.3.1.42 0.065
-
 palmitoyl-CoA
-
 Cavia porcellus
2.3.1.42 0.1 0.5 dihydroxyacetone phosphate in presence of asolectin Cavia porcellus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.42 peroxisome
-
 Cavia porcellus 5777
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.42 additional information no activation by Ca2+ Cavia porcellus
2.3.1.42 additional information no activation by Mg2+, partially purified Cavia porcellus
2.3.1.42 additional information no activation by Mn2+, partially purified Cavia porcellus

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.42 Cavia porcellus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.42 partial, solubilized with sodium cholate-KCl Cavia porcellus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.42 liver
-
 Cavia porcellus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.42 0.106
-
-
 Cavia porcellus
2.3.1.42 0.212
-
-
 Cavia porcellus

Storage Stability

EC Number Storage Stability Organism
2.3.1.42 2-4°C, 30-50% loss of activity after 1 month, DTT stabilizes during storage Cavia porcellus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.42 acyl-CoA + dihydroxyacetone phosphate i.e. glycerone phosphate, saturated acyl-CoAs preferred over unsaturated Cavia porcellus CoA + acyldihydroxyacetone phosphate i.e. acylglycerone phosphate r
2.3.1.42 arachidonoyl-CoA + dihydroxyacetone phosphate i.e. eicosatetraenoyl-CoA, poor substrate Cavia porcellus CoA + arachidonoyldihydroxyacetone phosphate
-
 r
2.3.1.42 linoleoyl-CoA + dihydroxyacetone phosphate
-
 Cavia porcellus CoA + linoleoyldihydroxyacetone phosphate
-
 r
2.3.1.42 oleoyl-CoA + dihydroxyacetone phosphate poor substrate Cavia porcellus CoA + oleoyldihydroxyacetone phosphate
-
 r
2.3.1.42 palmitoyl-CoA + dihydroxyacetone phosphate best substrate Cavia porcellus CoA + monopalmitoyldihydroxyacetone phosphate
-
 r
2.3.1.42 stearoyl-CoA + dihydroxyacetone phosphate acylation at 35% the rate of palmitoyl-CoA Cavia porcellus CoA + stearoyldihydroxyacetone phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.42 37
-
 assay at Cavia porcellus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.42 48
-
 t1/2: 15 min Cavia porcellus
2.3.1.42 51
-
 t1/2: 15 min, with asolectin Cavia porcellus
2.3.1.42 60
-
 10 min stable Cavia porcellus
2.3.1.42 60
-
 intact peroxisomes Cavia porcellus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.42 6.4
-
 in presence of asolectin Cavia porcellus
2.3.1.42 7 8.2 broad Cavia porcellus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.1.42 6 9 about half-maximal activity at pH 6.0 and pH 9.0, in the presence of asolectin Cavia porcellus