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Literature summary extracted from

  • McGuire, K.A.; Siggaard-Andersen, M.; Bangera, M.G.; Olsen, J.G.; von Wettstein-Knowles, P.
    beta-Ketoacyl-[acyl carrier protein] synthase I of Escherichia coli: aspects of the condensation mechanism revealed by analyses of mutations in the active site pocket (2001), Biochemistry, 40, 9836-9845.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.41 expression of synthase I and mutants as His-tagged proteins in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.41 C163A synthase I, site-directed mutagenesis, active site mutant, no activity, but decarboxylates malonyl-[acyl-carrier-protein] Escherichia coli
2.3.1.41 C163S synthase I, site-directed mutagenesis, active site mutant, increased activity, decarboxylates malonyl-[acyl-carrier-protein] Escherichia coli
2.3.1.41 D306A synthase I, site-directed mutagenesis, active site mutant, increased activity Escherichia coli
2.3.1.41 E309A synthase I, site-directed mutagenesis, active site mutant, reduced activity Escherichia coli
2.3.1.41 H298A synthase I, site-directed mutagenesis, active site mutant, reduced activity Escherichia coli
2.3.1.41 H333A synthase I, site-directed mutagenesis, active site mutant, increased activity Escherichia coli
2.3.1.41 K328A synthase I, site-directed mutagenesis, active site mutant, reduced activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 [Acyl-carrier-protein] wild-type synthase I and mutants, not mutants C163S and K328A, overview Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.41 additional information
-
 synthase I mutants Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Escherichia coli
-
 synthase I
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41 recombinant synthase I and mutants as His-tagged protein from E. coli Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.41 an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] mechanism and active site structure and function Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 malonyl-[acyl-carrier protein] wild-type synthase I and mutants C163A and C163S Escherichia coli acetyl-[acyl-carrier protein] + CO2
-
 ?
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Escherichia coli 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?
2.3.1.41 tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] synthase I Escherichia coli 3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.41 More synthase I mutants Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.41 20
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.41 6.8
-
 assay at Escherichia coli