EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.41 | plasmid-based expression of synthase III wild-type and mutants in Streptomyces glaucescens and His-tagged in Escherichia coli | Streptomyces glaucescens |
2.3.1.180 | subcloning in Escherichia coli strain TG-2, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Streptomyces glaucescens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.41 | C122A | site-directed mutagenesis, 75% enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type | Streptomyces glaucescens |
2.3.1.41 | C122Q | site-directed mutagenesis, 500% enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type | Streptomyces glaucescens |
2.3.1.41 | C122S | site-directed mutagenesis, enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type | Streptomyces glaucescens |
2.3.1.180 | C122A | site-directed mutagenesis, the mutant enzyme expressed in Streptomyces glaucescens generates 75% more straight-chain fatty acids than the wild-type enzyme, plasmid-based expression does not affect Escherichia coli strain TG-2, the mutation causes uncoupling of condensation and decarboxylation reactions | Streptomyces glaucescens |
2.3.1.180 | C122Q | site-directed mutagenesis, the mutant enzyme expressed in Streptomyces glaucescens generates 500% more straight-chain fatty acids than the wild-type enzyme, plasmid-based expression does not affect Escherichia coli strain TG-2, the mutation causes uncoupling of condensation and decarboxylation reactions | Streptomyces glaucescens |
2.3.1.180 | C122S | site-directed mutagenesis, the mutant enzyme expressed in Streptomyces glaucescens generates 100% more straight-chain fatty acids than the wild-type enzyme, plasmid-based expression does not affect Escherichia coli strain TG-2, the mutation causes uncoupling of condensation and decarboxylation reactions | Streptomyces glaucescens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.41 | butyryl-CoA | synthase III mutants C122Aand C122Q: inhibition of decarboxylation activity in presence of | Streptomyces glaucescens | |
2.3.1.41 | isobutyryl-CoA | synthase III mutants C122Aand C122Q: inhibition of decarboxylation activity in presence of | Streptomyces glaucescens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Streptomyces glaucescens | initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.180 | acetyl-CoA + malonyl-[acyl-carrier protein] | Streptomyces glaucescens | the enzyme initiates the fatty acid biosynthesis, overview | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.41 | Streptomyces glaucescens | - |
synthase III, gene fabH | - |
2.3.1.180 | Streptomyces glaucescens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.41 | recombinant from E. coli as His-tagged protein | Streptomyces glaucescens |
2.3.1.41 | wild-type and mutants | Streptomyces glaucescens |
2.3.1.180 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Streptomyces glaucescens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | active site cysteine | Streptomyces glaucescens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo | Streptomyces glaucescens | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.41 | isobutyryl-CoA + malonyl-[acyl-carrier protein] | synthase III | Streptomyces glaucescens | ? | - |
? | |
2.3.1.41 | isobutyryl-CoA + malonyl-[acyl-carrier protein] | isobutyryl-CoA is a precursor of branched-chain fatty acid synthesis | Streptomyces glaucescens | ? | - |
? | |
2.3.1.41 | malonyl-[acyl-carrier protein] | - |
Streptomyces glaucescens | acetyl-[acyl-carrier protein] + CO2 | - |
? | |
2.3.1.41 | saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | substrate specificity, overview | Streptomyces glaucescens | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.41 | saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | synthase III, broad specificity | Streptomyces glaucescens | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.180 | acetyl-CoA + malonyl-[acyl-carrier protein] | - |
Streptomyces glaucescens | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? | |
2.3.1.180 | acetyl-CoA + malonyl-[acyl-carrier protein] | the enzyme initiates the fatty acid biosynthesis, overview | Streptomyces glaucescens | acetoacetyl-[acyl-carrier protein] + CoA + CO2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.41 | KAS | - |
Streptomyces glaucescens |
2.3.1.180 | ACO synthase III | - |
Streptomyces glaucescens |
2.3.1.180 | beta-ketoacyl-acyl carrier protein synthase III | - |
Streptomyces glaucescens |
2.3.1.180 | KASIII | - |
Streptomyces glaucescens |