EC Number | Application | Comment | Organism |
---|---|---|---|
2.3.1.41 | medicine | target for the development of drugs for the treatment of cancer and tuberculosis | Synechocystis sp. |
2.3.1.41 | nutrition | target for the engineering of plant seed oils | Synechocystis sp. |
2.3.1.41 | pharmacology | target for the development of drugs for the treatment of cancer and tuberculosis, involved in biosynthesis of precursors of pharmacological agents | Synechocystis sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.41 | expression of wild-type and mutant synthase II in Escherichia coli | Synechocystis sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.41 | A193M | site-directed mutagenesis, hydrophobic acyl-binding pocket is decreased in size by 2.55fold, 12fold reduced catalytic activity | Synechocystis sp. |
2.3.1.41 | I108F | site-directed mutagenesis, hydrophobic acyl-binding pocket is decreased in size by 1.75fold, 38fold reduced catalytic activity towards octanoyl-[acyl-carrier-protein] compared to the activity towards hexanoy-[acyl-carrier-protein] | Synechocystis sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.41 | cerulenin | 12-carbon mycotoxin, irreversible inhibition, binding motif; at 0.05 mM: wild-type is completely inactivated, mutant I108F and A193M show only 10% reduced activity | Synechocystis sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.41 | Synechocystis sp. | - |
synthase II | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.41 | synthase II, recombinant from E. coli | Synechocystis sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.41 | an acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | active site cysteine | Synechocystis sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.41 | hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Synechocystis sp. | 3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.41 | octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Synechocystis sp. | 3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.41 | saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | - |
Synechocystis sp. | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.41 | KAS | - |
Synechocystis sp. |