EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.41 | synthase III, overexpression in Escherichia coli strain JM101, construction of binary expression vectors for transformation of Brassica napus | Escherichia coli |
2.3.1.180 | overexpression in strain JM101 inhibits bacterial cell growth and alters the membrane fatty acid composition, overexpression of the chimeric enzyme possessing a transit peptide in Brassica napus seeds and cotyledons via Agrobacterium transfection | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.41 | additional information | transformation of Brassica napus via Agrobacterium tumefaciens infection with binary vector overexpressing Escherichia coli fabH gene, targeted expression in cytoplasm and plastids is seed specific, modified fatty acid profile of seed oil | Escherichia coli |
2.3.1.41 | additional information | transformation of Brassica napus via Agrobacterium tumefaciens infection with binary vector overexpressing Escherichia coli fabH gene, targeted expression in cytoplasm and plastids is seed specific, modified fatty acid profile of seed oil | Brassica napus |
2.3.1.180 | additional information | construction of transgenic Brassica napus plants overexpressing the Escherichia coli enzyme in seeds, expression analysis during seed adevelopment | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.180 | additional information | - |
additional information | kinetics | Escherichia coli | |
2.3.1.180 | 0.045 | - |
acetyl-CoA | pH 7.0, 25°C, recombinant enzyme from Brassica napus seeds | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Escherichia coli | involved in biosynthesis of unsaturated and saturated fatty acids | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | Brassica napus | involved in biosynthesis of unsaturated and saturated fatty acids | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.41 | Brassica napus | - |
cv. Westar | - |
2.3.1.41 | Escherichia coli | - |
synthase III, gene fabH | - |
2.3.1.180 | Escherichia coli | - |
gene fabH | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.3.1.41 | additional information | - |
wild-type and transgenic Brassica napus plants, expressing the E. coli synthase III | Escherichia coli |
2.3.1.41 | additional information | - |
wild-type and transgenic Brassica napus plants, expressing the E. coli synthase III | Brassica napus |
2.3.1.180 | additional information | - |
recombinant enzyme in Brassica napus seeds | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | involved in biosynthesis of unsaturated and saturated fatty acids | Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.41 | acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | involved in biosynthesis of unsaturated and saturated fatty acids | Brassica napus | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.41 | saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | fatty acid composition of Brassica napus wild-type and transgenic plants overexpressing the E. coli synthase III | Escherichia coli | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? | |
2.3.1.41 | saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] | fatty acid composition of Brassica napus wild-type and transgenic plants overexpressing the E. coli synthase III | Brassica napus | 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein] | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.41 | KAS | - |
Brassica napus |
2.3.1.180 | 3-ketoacyl-acyl carrier protein synthase III | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.180 | 25 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.180 | 7 | - |
assay at | Escherichia coli |