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Literature summary extracted from

  • Clough, R.; Matthis, A.L.; Barnum, S.R.; Jaworski, J.G.
    Purification and characterization of 3-ketoacyl-acyl carrier protein synthase III from spinach. A condensing enzyme utilizing acetyl-coenzyme A to initiate fatty acid synthesis (1992), J. Biol. Chem., 267, 20992-20998.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.3.1.41 dithiothreitol stabilizes Spinacia oleracea
2.3.1.41 glycerol stabilizes Spinacia oleracea
2.3.1.41 Triton X-100 stabilizes Spinacia oleracea
2.3.1.180 0.01% Triton X-100 is essential for enzyme stability during purification Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.41 arsenite synthase III Spinacia oleracea
2.3.1.41 N-ethylmaleimide
-
 Spinacia oleracea
2.3.1.180 arsenite 45% inhibition at 5 mM, 98% inhibition at 20 mM Spinacia oleracea
2.3.1.180 N-ethylmaleimide complete inhibition at 5 mM Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.41 40500
-
 2 * 40500, synthase III, SDS-PAGE Spinacia oleracea
2.3.1.41 63000
-
 synthase III, gel filtration Spinacia oleracea
2.3.1.180 40500
-
 2 * 40500, SDS-PAGE Spinacia oleracea
2.3.1.180 63000
-
 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Spinacia oleracea
-
 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.41 Spinacia oleracea
-
 synthase III
-
2.3.1.180 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.41 synthase III, native wild-type and recombinant from E. coli E103S Spinacia oleracea
2.3.1.180 3500fold to homogeneity from leaves in an eight-step procedure including ACP affinity chromatography Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.41 leaf
-
 Spinacia oleracea
-
2.3.1.180 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.41 additional information
-
 comparison of activity, recombinant and native enzyme Spinacia oleracea
2.3.1.41 7
-
 purified native enzyme Spinacia oleracea
2.3.1.180 7
-
 purified enzyme Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.3.1.41 -80°C, several weeks in the presence of 20% v/v glycerol, 0.1% Triton X-100 and dithiothreitol Spinacia oleracea
2.3.1.180 -80°C, purified enzyme, 20 mM Tris buffer, 1 mM DTT, 20% glycerol, 0.01% Triton X-100, several weeks with slight loss of activity Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.41 acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] synthase III, recombinant enzyme from E. coli is absolutely specific for Spinacia oleracea acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 r
2.3.1.41 saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
 Spinacia oleracea 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
 r
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Spinacia oleracea acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 r
2.3.1.180 additional information substrate specificity, the enzyme also shows acetyltransferase activity, which is lower than the 3-ketoacyl carrier protein synthase activity Spinacia oleracea ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.41 dimer 2 * 40500, synthase III, SDS-PAGE Spinacia oleracea
2.3.1.180 dimer 2 * 40500, SDS-PAGE Spinacia oleracea

Synonyms

EC Number Synonyms Comment Organism
2.3.1.41 3-ketoacyl-ACP synthase
-
 Spinacia oleracea
2.3.1.41 KAS
-
 Spinacia oleracea
2.3.1.180 3-ketoacyl carrier protein synthase III
-
 Spinacia oleracea
2.3.1.180 KAS-III
-
 Spinacia oleracea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.180 32
-
 assay at Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.180 8
-
 assay at Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.180 CoA
-
 Spinacia oleracea