BRENDA - Enzyme Database show

Isolation and function of spinach leaf beta-ketoacyl-[acyl-carrier-protein] synthases

Shimakata, T.; Stumpf, P.L.; Proc. Natl. Acad. Sci. USA 79, 5808-5812 (1982)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.41
arsenite
synthase II and I
Spinacia oleracea
2.3.1.41
cerulenin
-
Spinacia oleracea
2.3.1.41
N-ethylmaleimide
5 mM
Spinacia oleracea
2.3.1.41
p-chloromercuribenzoate
complete inhibition at 1 mM
Spinacia oleracea
2.3.1.179
arsenite
1 mM, 42% inhibition
Spinacia oleracea
2.3.1.179
cerulenin
0.05 mM, 50% inhibition
Spinacia oleracea
2.3.1.179
NEM
5 mM, complete inhibition
Spinacia oleracea
2.3.1.179
PCMB
1 mM, complete inhibition
Spinacia oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.41
0.0036
-
palmitoyl-[acyl-carrier protein]
synthase II
Spinacia oleracea
2.3.1.41
0.0055
-
malonyl-CoA
-
Spinacia oleracea
2.3.1.41
0.0133
-
decanoyl-[acyl-carrier protein]
synthase II
Spinacia oleracea
2.3.1.41
0.0139
-
tetradecanoyl-[acyl-carrier protein]
synthase II
Spinacia oleracea
2.3.1.179
0.0036
-
palmitoyl-[acyl-carrier protein]
-
Spinacia oleracea
2.3.1.179
0.0055
-
malonyl-CoA
-
Spinacia oleracea
2.3.1.179
0.0133
-
decanoyl-[acyl-carrier protein]
-
Spinacia oleracea
2.3.1.179
0.0139
-
myristoyl-[acyl-carrier protein]
-
Spinacia oleracea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.41
56000
-
synthase I, gel filtration
Spinacia oleracea
2.3.1.41
57500
-
synthase II, gel filtration
Spinacia oleracea
2.3.1.179
56000
-
gel filtration
Spinacia oleracea
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.41
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
Spinacia oleracea
part of non-associated fatty acid synthase system of plants and prokaryotes
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
?
2.3.1.41
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
Spinacia oleracea
involved in biosynthesis of saturated fatty acids
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
?
2.3.1.179
additional information
Spinacia oleracea
the enzyme plays a key role in synthesis of C18 fatty acids
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.41
Spinacia oleracea
-
synthase I and II
-
2.3.1.179
Spinacia oleracea
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.41
synthases II
Spinacia oleracea
2.3.1.179
-
Spinacia oleracea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.41
leaf
-
Spinacia oleracea
-
2.3.1.179
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.41
additional information
-
-
Spinacia oleracea
2.3.1.179
0.023
-
-
Spinacia oleracea
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.41
-70°C, at least 1 month
Spinacia oleracea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.41
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
synthase I
486924
Spinacia oleracea
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
part of non-associated fatty acid synthase system of plants and prokaryotes
486924
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
involved in biosynthesis of saturated fatty acids
486924
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
synthase I
486924
Spinacia oleracea
dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
only synthase II, best substrate
486924
Spinacia oleracea
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
i.e. palmitoyl-[acyl-carrier-protein]
486924
Spinacia oleracea
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
best substrate, synthase I
486924
Spinacia oleracea
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
additional information
synthase I is specific for short-chain fatty acid prolongation while synthase II is active in C18 formation from C16
486924
Spinacia oleracea
?
-
-
-
-
2.3.1.41
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
substrate specificity, overview
486924
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
486924
Spinacia oleracea
?
2.3.1.41
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
not octodecanoyl-[acyl-carrier-protein], i.e stearoyl-[acyl-carrier-protein]
486924
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
486924
Spinacia oleracea
?
2.3.1.41
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
i.e. myristoyl-[acyl-carrier-protein], only synthase II
486924
Spinacia oleracea
3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.179
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
486924
Spinacia oleracea
? + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.179
additional information
the enzyme plays a key role in synthesis of C18 fatty acids
486924
Spinacia oleracea
?
-
-
-
-
2.3.1.179
additional information
enzyme is inactive with stearoyl-[acyl-carrier-protein]
486924
Spinacia oleracea
?
-
-
-
-
2.3.1.179
myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
486924
Spinacia oleracea
? + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.179
palmitoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
486924
Spinacia oleracea
? + CO2 + [acyl-carrier protein]
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.41
8.1
8.5
-
Spinacia oleracea
2.3.1.179
8.1
8.5
-
Spinacia oleracea
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.41
6.8
9.1
-
Spinacia oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.41
arsenite
synthase II and I
Spinacia oleracea
2.3.1.41
cerulenin
-
Spinacia oleracea
2.3.1.41
N-ethylmaleimide
5 mM
Spinacia oleracea
2.3.1.41
p-chloromercuribenzoate
complete inhibition at 1 mM
Spinacia oleracea
2.3.1.179
arsenite
1 mM, 42% inhibition
Spinacia oleracea
2.3.1.179
cerulenin
0.05 mM, 50% inhibition
Spinacia oleracea
2.3.1.179
NEM
5 mM, complete inhibition
Spinacia oleracea
2.3.1.179
PCMB
1 mM, complete inhibition
Spinacia oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.41
0.0036
-
palmitoyl-[acyl-carrier protein]
synthase II
Spinacia oleracea
2.3.1.41
0.0055
-
malonyl-CoA
-
Spinacia oleracea
2.3.1.41
0.0133
-
decanoyl-[acyl-carrier protein]
synthase II
Spinacia oleracea
2.3.1.41
0.0139
-
tetradecanoyl-[acyl-carrier protein]
synthase II
Spinacia oleracea
2.3.1.179
0.0036
-
palmitoyl-[acyl-carrier protein]
-
Spinacia oleracea
2.3.1.179
0.0055
-
malonyl-CoA
-
Spinacia oleracea
2.3.1.179
0.0133
-
decanoyl-[acyl-carrier protein]
-
Spinacia oleracea
2.3.1.179
0.0139
-
myristoyl-[acyl-carrier protein]
-
Spinacia oleracea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.41
56000
-
synthase I, gel filtration
Spinacia oleracea
2.3.1.41
57500
-
synthase II, gel filtration
Spinacia oleracea
2.3.1.179
56000
-
gel filtration
Spinacia oleracea
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.3.1.41
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
Spinacia oleracea
part of non-associated fatty acid synthase system of plants and prokaryotes
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
?
2.3.1.41
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
Spinacia oleracea
involved in biosynthesis of saturated fatty acids
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
?
2.3.1.179
additional information
Spinacia oleracea
the enzyme plays a key role in synthesis of C18 fatty acids
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.41
synthases II
Spinacia oleracea
2.3.1.179
-
Spinacia oleracea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.41
leaf
-
Spinacia oleracea
-
2.3.1.179
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.3.1.41
additional information
-
-
Spinacia oleracea
2.3.1.179
0.023
-
-
Spinacia oleracea
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.41
-70°C, at least 1 month
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.41
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
synthase I
486924
Spinacia oleracea
acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
part of non-associated fatty acid synthase system of plants and prokaryotes
486924
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
involved in biosynthesis of saturated fatty acids
486924
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
synthase I
486924
Spinacia oleracea
dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
only synthase II, best substrate
486924
Spinacia oleracea
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
i.e. palmitoyl-[acyl-carrier-protein]
486924
Spinacia oleracea
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
best substrate, synthase I
486924
Spinacia oleracea
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
additional information
synthase I is specific for short-chain fatty acid prolongation while synthase II is active in C18 formation from C16
486924
Spinacia oleracea
?
-
-
-
-
2.3.1.41
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
substrate specificity, overview
486924
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
486924
Spinacia oleracea
?
2.3.1.41
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
not octodecanoyl-[acyl-carrier-protein], i.e stearoyl-[acyl-carrier-protein]
486924
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
486924
Spinacia oleracea
?
2.3.1.41
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
i.e. myristoyl-[acyl-carrier-protein], only synthase II
486924
Spinacia oleracea
3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.179
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
486924
Spinacia oleracea
? + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.179
additional information
the enzyme plays a key role in synthesis of C18 fatty acids
486924
Spinacia oleracea
?
-
-
-
-
2.3.1.179
additional information
enzyme is inactive with stearoyl-[acyl-carrier-protein]
486924
Spinacia oleracea
?
-
-
-
-
2.3.1.179
myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
486924
Spinacia oleracea
? + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.179
palmitoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
486924
Spinacia oleracea
? + CO2 + [acyl-carrier protein]
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.3.1.41
8.1
8.5
-
Spinacia oleracea
2.3.1.179
8.1
8.5
-
Spinacia oleracea
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.41
6.8
9.1
-
Spinacia oleracea