BRENDA - Enzyme Database show

Purification and characterization of beta-ketoacyl-[acyl-carrier-protein] synthetase I from Spinacia oleracea leaves

Shimakata, T.; Stumpf, P.L.; Arch. Biochem. Biophys. 220, 39-45 (1983)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.41
arsenite
weak at 1 mM
Spinacia oleracea
2.3.1.41
cerulenin
antibiotic, protection by hexanoyl-[acyl-carrier-protein], decanoyl-[acyl-carrier-protein], and tetradecanoyl-[acyl-carrier-protein]
Spinacia oleracea
2.3.1.41
N-ethylmaleimide
5 mM
Spinacia oleracea
2.3.1.41
p-chloromercuribenzoate
complete inhibition at 1 mM
Spinacia oleracea
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.41
additional information
-
additional information
-
Spinacia oleracea
2.3.1.41
0.004
-
malonyl-CoA
in presence of [acyl-carrier-protein]
Spinacia oleracea
2.3.1.41
0.0075
-
hexanoyl-[acyl-carrier protein]
-
Spinacia oleracea
2.3.1.41
0.0091
-
tetradecanoyl-[acyl-carrier protein]
-
Spinacia oleracea
2.3.1.41
0.016
-
malonyl-CoA
without [acyl-carrier-protein]
Spinacia oleracea
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.41
56000
-
synthase I, gel filtration
Spinacia oleracea
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.3.1.41
Spinacia oleracea
-
synthase I and II
-
Purification (Commentary)
EC Number
Commentary
Organism
2.3.1.41
synthase I
Spinacia oleracea
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.41
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.41
0.0083
-
purified enzyme
Spinacia oleracea
Storage Stability
EC Number
Storage Stability
Organism
2.3.1.41
-20░C, t1/2: 1 month
Spinacia oleracea
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.41
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
486920
Spinacia oleracea
dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
poor substrate
486920
Spinacia oleracea
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
i.e. palmitoyl-[acyl-carrier-protein]
486920
Spinacia oleracea
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
best substrate, synthase I
486920
Spinacia oleracea
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
substrate specificity, overview
486920
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
486920
Spinacia oleracea
?
2.3.1.41
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
not octodecanoyl-[acyl-carrier-protein], i.e stearoyl-[acyl-carrier-protein]
486920
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
486920
Spinacia oleracea
?
2.3.1.41
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
486920
Spinacia oleracea
3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
-
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.3.1.41
25
-
assay at
Spinacia oleracea
2.3.1.41
37
-
assay at
Spinacia oleracea
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.41
6
8.2
-
Spinacia oleracea
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.3.1.41
arsenite
weak at 1 mM
Spinacia oleracea
2.3.1.41
cerulenin
antibiotic, protection by hexanoyl-[acyl-carrier-protein], decanoyl-[acyl-carrier-protein], and tetradecanoyl-[acyl-carrier-protein]
Spinacia oleracea
2.3.1.41
N-ethylmaleimide
5 mM
Spinacia oleracea
2.3.1.41
p-chloromercuribenzoate
complete inhibition at 1 mM
Spinacia oleracea
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.3.1.41
additional information
-
additional information
-
Spinacia oleracea
2.3.1.41
0.004
-
malonyl-CoA
in presence of [acyl-carrier-protein]
Spinacia oleracea
2.3.1.41
0.0075
-
hexanoyl-[acyl-carrier protein]
-
Spinacia oleracea
2.3.1.41
0.0091
-
tetradecanoyl-[acyl-carrier protein]
-
Spinacia oleracea
2.3.1.41
0.016
-
malonyl-CoA
without [acyl-carrier-protein]
Spinacia oleracea
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.3.1.41
56000
-
synthase I, gel filtration
Spinacia oleracea
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.3.1.41
synthase I
Spinacia oleracea
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.3.1.41
leaf
-
Spinacia oleracea
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.3.1.41
0.0083
-
purified enzyme
Spinacia oleracea
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.3.1.41
-20░C, t1/2: 1 month
Spinacia oleracea
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.3.1.41
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
486920
Spinacia oleracea
dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
poor substrate
486920
Spinacia oleracea
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
i.e. palmitoyl-[acyl-carrier-protein]
486920
Spinacia oleracea
3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
best substrate, synthase I
486920
Spinacia oleracea
3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
?
2.3.1.41
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
substrate specificity, overview
486920
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
486920
Spinacia oleracea
?
2.3.1.41
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
not octodecanoyl-[acyl-carrier-protein], i.e stearoyl-[acyl-carrier-protein]
486920
Spinacia oleracea
3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
486920
Spinacia oleracea
?
2.3.1.41
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]
-
486920
Spinacia oleracea
3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]
-
-
-
-
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.3.1.41
25
-
assay at
Spinacia oleracea
2.3.1.41
37
-
assay at
Spinacia oleracea
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.3.1.41
6
8.2
-
Spinacia oleracea